1llo: Difference between revisions

Line 1:

[[Image:1llo.gif|left|200px]]<br /><applet load="1llo" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1llo.gif|left|200px]]<br /><applet load="1llo" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1llo, resolution 1.85Å" />

'''HEVAMINE A (A PLANT ENDOCHITINASE/LYSOZYME) COMPLEXED WITH ALLOSAMIDIN'''<br />

==Overview==

The plant enzyme hevamine has both chitinase and lysozyme activity. HPLC, analysis of the products of the hydrolysis of chitopentaose shows that, hevamine acts with retention of the configuration, despite the absence of, a nucleophilic or stabilizing carboxylate. To analyze the stabilization of, a putative oxocarbonium ion intermediate, the X-ray structure of hevamine, complexed with the inhibitor allosamidin was determined at 1.85 A, resolution. This structure supports the role of Glu127 as a proton donor., The allosamizoline group binds in the center of the active site, mimicking, a reaction intermediate in which a positive charge at C1 is stabilized, intramolecularly by the carbonyl oxygen of the N-acetyl group at C2.

The plant enzyme hevamine has both chitinase and lysozyme activity. HPLC analysis of the products of the hydrolysis of chitopentaose shows that hevamine acts with retention of the configuration, despite the absence of a nucleophilic or stabilizing carboxylate. To analyze the stabilization of a putative oxocarbonium ion intermediate, the X-ray structure of hevamine complexed with the inhibitor allosamidin was determined at 1.85 A resolution. This structure supports the role of Glu127 as a proton donor. The allosamizoline group binds in the center of the active site, mimicking a reaction intermediate in which a positive charge at C1 is stabilized intramolecularly by the carbonyl oxygen of the N-acetyl group at C2.

==About this Structure==

1LLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis] with AMI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LLO OCA].

1LLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis] with <scene name='pdbligand=AMI:'>AMI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LLO OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Armand, S.]]

[[Category: Dijkstra, B.W.]]

[[Category: Dijkstra, B W.]]

[[Category: Henrissat, B.]]

[[Category: Isogai, A.]]

[[Category: Kalk, K.H.]]

[[Category: Kalk, K H.]]

[[Category: Scheltinga, A.C.Terwisscha.Van.]]

[[Category: Scheltinga, A C.Terwisscha Van.]]

[[Category: AMI]]

[[Category: chitinase]]

[[Category: lysozyme]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 20~~:39:~~32 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:06 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1llo.gif|left|200px]]<br /><applet load="1llo" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1llo.gif|left|200px]]<br /><applet load="1llo" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1llo, resolution 1.85&Aring;" />
 '''HEVAMINE A (A PLANT ENDOCHITINASE/LYSOZYME) COMPLEXED WITH ALLOSAMIDIN'''<br />
 ==Overview==
-The plant enzyme hevamine has both chitinase and lysozyme activity. HPLC, analysis of the products of the hydrolysis of chitopentaose shows that, hevamine acts with retention of the configuration, despite the absence of, a nucleophilic or stabilizing carboxylate. To analyze the stabilization of, a putative oxocarbonium ion intermediate, the X-ray structure of hevamine, complexed with the inhibitor allosamidin was determined at 1.85 A, resolution. This structure supports the role of Glu127 as a proton donor., The allosamizoline group binds in the center of the active site, mimicking, a reaction intermediate in which a positive charge at C1 is stabilized, intramolecularly by the carbonyl oxygen of the N-acetyl group at C2.
+The plant enzyme hevamine has both chitinase and lysozyme activity. HPLC analysis of the products of the hydrolysis of chitopentaose shows that hevamine acts with retention of the configuration, despite the absence of a nucleophilic or stabilizing carboxylate. To analyze the stabilization of a putative oxocarbonium ion intermediate, the X-ray structure of hevamine complexed with the inhibitor allosamidin was determined at 1.85 A resolution. This structure supports the role of Glu127 as a proton donor. The allosamizoline group binds in the center of the active site, mimicking a reaction intermediate in which a positive charge at C1 is stabilized intramolecularly by the carbonyl oxygen of the N-acetyl group at C2.
 ==About this Structure==
-LLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis] with AMI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LLO OCA].
+LLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis] with <scene name='pdbligand=AMI:'>AMI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LLO OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Armand, S.]]
-[[Category: Dijkstra, B.W.]]
+[[Category: Dijkstra, B W.]]
 [[Category: Henrissat, B.]]
 [[Category: Isogai, A.]]
-[[Category: Kalk, K.H.]]
+[[Category: Kalk, K H.]]
-[[Category: Scheltinga, A.C.Terwisscha.Van.]]
+[[Category: Scheltinga, A C.Terwisscha Van.]]
 [[Category: AMI]]
 [[Category: chitinase]]
 [[Category: lysozyme]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:39:32 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:06 2008''