1log: Difference between revisions

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[[Image:1log.jpg|left|200px]]<br /><applet load="1log" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1log.jpg|left|200px]]<br /><applet load="1log" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1log, resolution 2.1Å" />

'''X-RAY STRUCTURE OF A (ALPHA-MAN(1-3)BETA-MAN(1-4)GLCNAC)-LECTIN COMPLEX AT 2.1 ANGSTROMS RESOLUTION'''<br />

==Overview==

We describe herein the high resolution refined x-ray structure of a, trisaccharide, which is a part of the N-acetyllactosamine type glycan, found in the majority of the N-glycosyl-proteins, complexed to the, isolectin I. According to the potentials used by Imberty et al. (Imburty, A., Gerber, S., Tran, V., and Perez, S. (1990) Glycoconjugate J. 7, 27-54), the trisaccharide is in a low-energy state. Only one mannose moiety, establishes direct hydrogen bonds with the lectin, as it is the case for, monosaccharide-lectin complexes. The comparison of our trisaccharide with, the one determined in solution by Warin et al. (Warin, V., Baert, F., Fouret, R., Strecker, G., Fournet, B., and Montreuil, J. (1979) Carbohydr., Res. 76, 11-22) shows that both adopt roughly the same conformation. The, differences in these two sugar structures allow us to assign the role of, water molecules present in the vicinity of our trisaccharide for the, stabilization of this sugar-lectin complex.

We describe herein the high resolution refined x-ray structure of a trisaccharide, which is a part of the N-acetyllactosamine type glycan found in the majority of the N-glycosyl-proteins, complexed to the isolectin I. According to the potentials used by Imberty et al. (Imburty, A., Gerber, S., Tran, V., and Perez, S. (1990) Glycoconjugate J. 7, 27-54) the trisaccharide is in a low-energy state. Only one mannose moiety establishes direct hydrogen bonds with the lectin, as it is the case for monosaccharide-lectin complexes. The comparison of our trisaccharide with the one determined in solution by Warin et al. (Warin, V., Baert, F., Fouret, R., Strecker, G., Fournet, B., and Montreuil, J. (1979) Carbohydr. Res. 76, 11-22) shows that both adopt roughly the same conformation. The differences in these two sugar structures allow us to assign the role of water molecules present in the vicinity of our trisaccharide for the stabilization of this sugar-lectin complex.

==About this Structure==

1LOG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus] with CA and MN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LOG OCA].

1LOG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LOG OCA].

==Reference==

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[[Category: lectin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 20~~:43:~~25 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:58 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1log.jpg|left|200px]]<br /><applet load="1log" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1log.jpg|left|200px]]<br /><applet load="1log" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1log, resolution 2.1&Aring;" />
 '''X-RAY STRUCTURE OF A (ALPHA-MAN(1-3)BETA-MAN(1-4)GLCNAC)-LECTIN COMPLEX AT 2.1 ANGSTROMS RESOLUTION'''<br />
 ==Overview==
-We describe herein the high resolution refined x-ray structure of a, trisaccharide, which is a part of the N-acetyllactosamine type glycan, found in the majority of the N-glycosyl-proteins, complexed to the, isolectin I. According to the potentials used by Imberty et al. (Imburty, A., Gerber, S., Tran, V., and Perez, S. (1990) Glycoconjugate J. 7, 27-54), the trisaccharide is in a low-energy state. Only one mannose moiety, establishes direct hydrogen bonds with the lectin, as it is the case for, monosaccharide-lectin complexes. The comparison of our trisaccharide with, the one determined in solution by Warin et al. (Warin, V., Baert, F., Fouret, R., Strecker, G., Fournet, B., and Montreuil, J. (1979) Carbohydr., Res. 76, 11-22) shows that both adopt roughly the same conformation. The, differences in these two sugar structures allow us to assign the role of, water molecules present in the vicinity of our trisaccharide for the, stabilization of this sugar-lectin complex.
+We describe herein the high resolution refined x-ray structure of a trisaccharide, which is a part of the N-acetyllactosamine type glycan found in the majority of the N-glycosyl-proteins, complexed to the isolectin I. According to the potentials used by Imberty et al. (Imburty, A., Gerber, S., Tran, V., and Perez, S. (1990) Glycoconjugate J. 7, 27-54) the trisaccharide is in a low-energy state. Only one mannose moiety establishes direct hydrogen bonds with the lectin, as it is the case for monosaccharide-lectin complexes. The comparison of our trisaccharide with the one determined in solution by Warin et al. (Warin, V., Baert, F., Fouret, R., Strecker, G., Fournet, B., and Montreuil, J. (1979) Carbohydr. Res. 76, 11-22) shows that both adopt roughly the same conformation. The differences in these two sugar structures allow us to assign the role of water molecules present in the vicinity of our trisaccharide for the stabilization of this sugar-lectin complex.
 ==About this Structure==
-LOG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus] with CA and MN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LOG OCA].
+LOG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lathyrus_ochrus Lathyrus ochrus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LOG OCA].
 ==Reference==
@@ Line 19: / Line 19: @@
 [[Category: lectin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:43:25 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:58 2008''