1lri: Difference between revisions
New page: left|200px<br /><applet load="1lri" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lri, resolution 1.45Å" /> '''BETA-CRYPTOGEIN-CHOL... |
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[[Image:1lri.gif|left|200px]]<br /><applet load="1lri" size=" | [[Image:1lri.gif|left|200px]]<br /><applet load="1lri" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1lri, resolution 1.45Å" /> | caption="1lri, resolution 1.45Å" /> | ||
'''BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX'''<br /> | '''BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic | Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic oomycete Phytophthora cryptogea. The protein also displays a sterol carrier activity. The native protein crystallizes in space group P4(1)22, with unit-cell parameters a = b = 46.51, c = 134.9 A (diffraction limit: 2.1 A). Its complex with cholesterol crystallizes in space group C222(1), with unit-cell parameters a = 30.96, b = 94.8, c = 65.3 A and a resolution enhanced to 1.45 A. The large inner non-specific hydrophobic cavity is able to accommodate a large variety of 3-beta-hydroxy sterols. Cryptogein probably acts as a sterol shuttle helping the pathogen to grow and complete its life cycle. | ||
==About this Structure== | ==About this Structure== | ||
1LRI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cryptogea Phytophthora cryptogea] with CL and CLR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1LRI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cryptogea Phytophthora cryptogea] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CLR:'>CLR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LRI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Phytophthora cryptogea]] | [[Category: Phytophthora cryptogea]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Blein, J | [[Category: Blein, J P.]] | ||
[[Category: Lascombe, M | [[Category: Lascombe, M B.]] | ||
[[Category: Milat, M | [[Category: Milat, M L.]] | ||
[[Category: Ponchet, M.]] | [[Category: Ponchet, M.]] | ||
[[Category: Prange, T.]] | [[Category: Prange, T.]] | ||
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[[Category: sterol carrier protein]] | [[Category: sterol carrier protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:47:42 2008'' | ||
Revision as of 11:47, 21 February 2008
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BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX
Overview
Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic oomycete Phytophthora cryptogea. The protein also displays a sterol carrier activity. The native protein crystallizes in space group P4(1)22, with unit-cell parameters a = b = 46.51, c = 134.9 A (diffraction limit: 2.1 A). Its complex with cholesterol crystallizes in space group C222(1), with unit-cell parameters a = 30.96, b = 94.8, c = 65.3 A and a resolution enhanced to 1.45 A. The large inner non-specific hydrophobic cavity is able to accommodate a large variety of 3-beta-hydroxy sterols. Cryptogein probably acts as a sterol shuttle helping the pathogen to grow and complete its life cycle.
About this Structure
1LRI is a Single protein structure of sequence from Phytophthora cryptogea with CL and CLR as ligands. Full crystallographic information is available from OCA.
Reference
The 1.45 A resolution structure of the cryptogein-cholesterol complex: a close-up view of a sterol carrier protein (SCP) active site., Lascombe MB, Ponchet M, Venard P, Milat ML, Blein JP, Prange T, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1442-7. Epub 2002, Aug 23. PMID:12198300
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