1lsp: Difference between revisions
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New page: left|200px<br /><applet load="1lsp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lsp, resolution 2.45Å" /> '''THE CRYSTAL STRUCTUR... |
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[[Image:1lsp.jpg|left|200px]]<br /><applet load="1lsp" size=" | [[Image:1lsp.jpg|left|200px]]<br /><applet load="1lsp" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1lsp, resolution 2.45Å" /> | caption="1lsp, resolution 2.45Å" /> | ||
'''THE CRYSTAL STRUCTURE OF A BULGECIN-INHIBITED G-TYPE LYSOZYME FROM THE EGG-WHITE OF THE AUSTRALIAN BLACK SWAN. A COMPARISON OF THE BINDING OF BULGECIN TO THREE MURAMIDASES'''<br /> | '''THE CRYSTAL STRUCTURE OF A BULGECIN-INHIBITED G-TYPE LYSOZYME FROM THE EGG-WHITE OF THE AUSTRALIAN BLACK SWAN. A COMPARISON OF THE BINDING OF BULGECIN TO THREE MURAMIDASES'''<br /> | ||
==Overview== | ==Overview== | ||
Bulgecin A, a bacterial metabolite, has been shown to bind in the | Bulgecin A, a bacterial metabolite, has been shown to bind in the active-site groove of the chicken-type lysozyme from the rainbow trout (RBTL) and in the lysozyme-like C-terminal domain, of a soluble lytic transglycosylase (C-SLT) from Escherichia coli. These enzymes are muramidases that cleave the glycosidic bonds in the glycan strands of the murein polymer. Here we report the crystal structure of a complex between the goose-type lysozyme from the egg white of the Australian black swan (SEWL) and bulgecin A at 2.45 A resolution. As is the case for the C-SLT/bulgecin and RBTL/bulgecin complexes, the ligand binds with the N-acetylglucosamine ring in subsite C and the proline moiety in site D where it interacts with the catalytic glutamic acid. The taurine residue interacts with the beta-sheet region. Comparisons of the three buigecin complexes show that the inhibitor has the same binding mode to the muramidases with similar protein-ligand interactions, particularly for SEWL and RBTL. From our results, it seems likely that bulgecin, in general, inhibits enzymes with lysozyme-like domains and thus might represent a novel class of natural antibiotics that act on murein-degrading rather than murein-synthesizing enzymes. | ||
==About this Structure== | ==About this Structure== | ||
1LSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cygnus_atratus Cygnus atratus] with BUL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | 1LSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cygnus_atratus Cygnus atratus] with <scene name='pdbligand=BUL:'>BUL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LSP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hough, E.]] | [[Category: Hough, E.]] | ||
[[Category: Isaacs, N | [[Category: Isaacs, N W.]] | ||
[[Category: Karlsen, S.]] | [[Category: Karlsen, S.]] | ||
[[Category: Rao, Z | [[Category: Rao, Z H.]] | ||
[[Category: BUL]] | [[Category: BUL]] | ||
[[Category: hydrolase (o-glycosyl)]] | [[Category: hydrolase (o-glycosyl)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:05 2008'' | ||
