1lun: Difference between revisions
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New page: left|200px<br /><applet load="1lun" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lun" /> '''NMR Structure of the Itk SH2 domain, Pro287t... |
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[[Image:1lun.gif|left|200px]]<br /><applet load="1lun" size=" | [[Image:1lun.gif|left|200px]]<br /><applet load="1lun" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''NMR Structure of the Itk SH2 domain, Pro287trans, energy minimized average structure'''<br /> | '''NMR Structure of the Itk SH2 domain, Pro287trans, energy minimized average structure'''<br /> | ||
==Overview== | ==Overview== | ||
Interleukin-2 tyrosine kinase (Itk) is a T cell-specific kinase required | Interleukin-2 tyrosine kinase (Itk) is a T cell-specific kinase required for a proper immune response following T cell receptor engagement. In addition to the kinase domain, Itk is composed of several noncatalytic regulatory domains, including a Src homology 2 (SH2) domain that contains a conformationally heterogeneous Pro residue. Cis-trans isomerization of a single prolyl imide bond within the SH2 domain mediates conformer-specific ligand recognition that may have functional implications in T cell signaling. To better understand the mechanism by which a proline switch regulates ligand binding, we have used NMR spectroscopy to determine two structures of Itk SH2 corresponding to the cis and trans imide bond-containing conformers. The structures indicate that the heterogeneous Pro residue acts as a hinge that modulates ligand recognition by controlling the relative orientation of protein-binding surfaces. | ||
==About this Structure== | ==About this Structure== | ||
1LUN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ACE and NH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http:// | 1LUN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUN OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
[[Category: Andreotti, A | [[Category: Andreotti, A H.]] | ||
[[Category: Brazin, K | [[Category: Brazin, K N.]] | ||
[[Category: Fulton, D | [[Category: Fulton, D B.]] | ||
[[Category: Mallis, R | [[Category: Mallis, R J.]] | ||
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: NH2]] | [[Category: NH2]] | ||
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[[Category: tsk]] | [[Category: tsk]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:36 2008'' | ||
