1lvb: Difference between revisions

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[[Image:1lvb.gif|left|200px]]<br /><applet load="1lvb" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1lvb.gif|left|200px]]<br /><applet load="1lvb" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1lvb, resolution 2.20Å" />

'''CATALYTICALLY INACTIVE TOBACCO ETCH VIRUS PROTEASE COMPLEXED WITH SUBSTRATE'''<br />

==Overview==

Because of its stringent sequence specificity, the 3C-type protease from, tobacco etch virus (TEV) is frequently used to remove affinity tags from, recombinant proteins. It is unclear, however, exactly how TEV protease, recognizes its substrates with such high selectivity. The crystal, structures of two TEV protease mutants, inactive C151A and, autolysis-resistant S219D, have now been solved at 2.2- and 1.8-A, resolution as complexes with a substrate and product peptide, respectively. The enzyme does not appear to have been perturbed by the, mutations in either structure, and the modes of binding of the product and, substrate are virtually identical. Analysis of the protein-ligand, interactions helps to delineate the structural determinants of substrate, specificity and provides guidance for reengineering the enzyme to further, improve its utility for biotechnological applications.

Because of its stringent sequence specificity, the 3C-type protease from tobacco etch virus (TEV) is frequently used to remove affinity tags from recombinant proteins. It is unclear, however, exactly how TEV protease recognizes its substrates with such high selectivity. The crystal structures of two TEV protease mutants, inactive C151A and autolysis-resistant S219D, have now been solved at 2.2- and 1.8-A resolution as complexes with a substrate and product peptide, respectively. The enzyme does not appear to have been perturbed by the mutations in either structure, and the modes of binding of the product and substrate are virtually identical. Analysis of the protein-ligand interactions helps to delineate the structural determinants of substrate specificity and provides guidance for reengineering the enzyme to further improve its utility for biotechnological applications.

==About this Structure==

1LVB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Tobacco_etch_virus Tobacco etch virus] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LVB OCA].

1LVB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Tobacco_etch_virus Tobacco etch virus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVB OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Tobacco etch virus]]

[[Category: Evdokimov, A.G.]]

[[Category: Evdokimov, A G.]]

[[Category: III, H.K.Peters.]]

[[Category: III, H K.Peters.]]

[[Category: Kapust, R.B.]]

[[Category: Kapust, R B.]]

[[Category: Li, M.]]

[[Category: Phan, J.]]

[[Category: Tropea, J.E.]]

[[Category: Tropea, J E.]]

[[Category: Waugh, D.S.]]

[[Category: Waugh, D S.]]

[[Category: Wlodawer, A.]]

[[Category: Zdanov, A.]]

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[[Category: protein-peptide complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 20~~:55:~~02 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:47 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1lvb.gif|left|200px]]<br /><applet load="1lvb" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lvb.gif|left|200px]]<br /><applet load="1lvb" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1lvb, resolution 2.20&Aring;" />
 '''CATALYTICALLY INACTIVE TOBACCO ETCH VIRUS PROTEASE COMPLEXED WITH SUBSTRATE'''<br />
 ==Overview==
-Because of its stringent sequence specificity, the 3C-type protease from, tobacco etch virus (TEV) is frequently used to remove affinity tags from, recombinant proteins. It is unclear, however, exactly how TEV protease, recognizes its substrates with such high selectivity. The crystal, structures of two TEV protease mutants, inactive C151A and, autolysis-resistant S219D, have now been solved at 2.2- and 1.8-A, resolution as complexes with a substrate and product peptide, respectively. The enzyme does not appear to have been perturbed by the, mutations in either structure, and the modes of binding of the product and, substrate are virtually identical. Analysis of the protein-ligand, interactions helps to delineate the structural determinants of substrate, specificity and provides guidance for reengineering the enzyme to further, improve its utility for biotechnological applications.
+Because of its stringent sequence specificity, the 3C-type protease from tobacco etch virus (TEV) is frequently used to remove affinity tags from recombinant proteins. It is unclear, however, exactly how TEV protease recognizes its substrates with such high selectivity. The crystal structures of two TEV protease mutants, inactive C151A and autolysis-resistant S219D, have now been solved at 2.2- and 1.8-A resolution as complexes with a substrate and product peptide, respectively. The enzyme does not appear to have been perturbed by the mutations in either structure, and the modes of binding of the product and substrate are virtually identical. Analysis of the protein-ligand interactions helps to delineate the structural determinants of substrate specificity and provides guidance for reengineering the enzyme to further improve its utility for biotechnological applications.
 ==About this Structure==
-LVB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Tobacco_etch_virus Tobacco etch virus] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LVB OCA].
+LVB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Tobacco_etch_virus Tobacco etch virus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVB OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Tobacco etch virus]]
-[[Category: Evdokimov, A.G.]]
+[[Category: Evdokimov, A G.]]
-[[Category: III, H.K.Peters.]]
+[[Category: III, H K.Peters.]]
-[[Category: Kapust, R.B.]]
+[[Category: Kapust, R B.]]
 [[Category: Li, M.]]
 [[Category: Phan, J.]]
-[[Category: Tropea, J.E.]]
+[[Category: Tropea, J E.]]
-[[Category: Waugh, D.S.]]
+[[Category: Waugh, D S.]]
 [[Category: Wlodawer, A.]]
 [[Category: Zdanov, A.]]
@@ Line 27: / Line 27: @@
 [[Category: protein-peptide complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:55:02 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:47 2008''