1gt9: Difference between revisions
New page: left|200px<br /> <applet load="1gt9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gt9, resolution 1.38Å" /> '''HIGH RESOLUTION CRY... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1GT9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_sp._mn-32 Bacillus sp. mn-32]] with CA and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GT9 OCA]]. | 1GT9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_sp._mn-32 Bacillus sp. mn-32]] with CA and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: CA1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GT9 OCA]]. | ||
==Reference== | ==Reference== | ||
| Line 28: | Line 28: | ||
[[Category: thermostable]] | [[Category: thermostable]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:18:21 2007'' | ||
Revision as of 11:13, 30 October 2007
|
HIGH RESOLUTION CRYSTAL STRUCTURE OF A THERMOSTABLE SERINE-CARBOXYL TYPE PROTEINASE
Overview
Kumamolysin is a thermostable endopeptidase from Bacillus novosp. MN-32, exhibiting maximal proteolytic activity around pH 3. It belongs to the, newly identified family of serine-carboxyl proteinases, which also, includes CLN2, a human lysosomal homolog recently implicated in a fatal, neurodegenerative disease. Kumamolysin and its complexes with two aldehyde, inhibitors were crystallized, and their three-dimensional structures were, solved and refined with X-ray data to 1.4 A resolution. As its Pseudomonas, homolog, kumamolysin exhibits a Ser/Glu/Asp catalytic triad with, particularly short interconnecting hydrogen bonds and an oxyanion hole, enabling the reactive serine to attack substrate peptide bonds at quite, acidic pH. An additional Glu/Trp pair, unique to kumamolysin, might, ... [(full description)]
About this Structure
1GT9 is a [Single protein] structure of sequence from [Bacillus sp. mn-32] with CA and SO4 as [ligands]. Structure known Active Site: CA1. Full crystallographic information is available from [OCA].
Reference
The 1.4 a crystal structure of kumamolysin: a thermostable serine-carboxyl-type proteinase., Comellas-Bigler M, Fuentes-Prior P, Maskos K, Huber R, Oyama H, Uchida K, Dunn BM, Oda K, Bode W, Structure. 2002 Jun;10(6):865-76. PMID:12057200
Page seeded by OCA on Tue Oct 30 13:18:21 2007
