1m3u: Difference between revisions
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New page: left|200px<br /><applet load="1m3u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m3u, resolution 1.800Å" /> '''Crystal Structure o... |
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[[Image:1m3u.gif|left|200px]]<br /><applet load="1m3u" size=" | [[Image:1m3u.gif|left|200px]]<br /><applet load="1m3u" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1m3u, resolution 1.800Å" /> | caption="1m3u, resolution 1.800Å" /> | ||
'''Crystal Structure of Ketopantoate Hydroxymethyltransferase complexed the Product Ketopantoate'''<br /> | '''Crystal Structure of Ketopantoate Hydroxymethyltransferase complexed the Product Ketopantoate'''<br /> | ||
==Overview== | ==Overview== | ||
We report the crystal structure of E. coli ketopantoate | We report the crystal structure of E. coli ketopantoate hydroxymethyltransferase (KPHMT) at 1.9 A resolution, in complex with its product, ketopantoate. KPHMT catalyzes the first step in the biosynthesis of pantothenate (vitamin B(5)), the precursor of coenzyme A and the acyl carrier protein cofactor. The structure of the decameric enzyme was solved by multiwavelength anomalous dispersion to locate 160 selenomethionine sites and phase 560 kDa of protein, making it the largest structure solved by this approach. KPHMT adopts the (betaalpha)(8) barrel fold and is a member of the phosphoenolpyruvate/pyruvate superfamily. The active site contains a ketopantoate bidentately coordinated to Mg(2+). Similar binding is likely for the substrate, alpha-ketoisovalerate, orienting the C3 for deprotonation. | ||
==About this Structure== | ==About this Structure== | ||
1M3U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and KPL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-methyl-2-oxobutanoate_hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.11 2.1.2.11] Full crystallographic information is available from [http:// | 1M3U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=KPL:'>KPL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-methyl-2-oxobutanoate_hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.11 2.1.2.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M3U OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Abell, C.]] | [[Category: Abell, C.]] | ||
[[Category: Blundell, T | [[Category: Blundell, T L.]] | ||
[[Category: Delft, F | [[Category: Delft, F von.]] | ||
[[Category: Dhanaraj, V.]] | [[Category: Dhanaraj, V.]] | ||
[[Category: Inoue, T.]] | [[Category: Inoue, T.]] | ||
[[Category: Ottenhof, H | [[Category: Ottenhof, H H.]] | ||
[[Category: Saldanha, S | [[Category: Saldanha, S A.]] | ||
[[Category: Smith, A | [[Category: Smith, A G.]] | ||
[[Category: Witty, M.]] | [[Category: Witty, M.]] | ||
[[Category: KPL]] | [[Category: KPL]] | ||
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[[Category: tim-barrel]] | [[Category: tim-barrel]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:08 2008'' | ||
