1m4n: Difference between revisions
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New page: left|200px<br /><applet load="1m4n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m4n, resolution 2.01Å" /> '''CRYSTAL STRUCTURE OF... |
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[[Image:1m4n.jpg|left|200px]]<br /><applet load="1m4n" size=" | [[Image:1m4n.jpg|left|200px]]<br /><applet load="1m4n" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1m4n, resolution 2.01Å" /> | caption="1m4n, resolution 2.01Å" /> | ||
'''CRYSTAL STRUCTURE OF APPLE ACC SYNTHASE IN COMPLEX WITH [2-(AMINO-OXY)ETHYL](5'-DEOXYADENOSIN-5'-YL)(METHYL)SULFONIUM'''<br /> | '''CRYSTAL STRUCTURE OF APPLE ACC SYNTHASE IN COMPLEX WITH [2-(AMINO-OXY)ETHYL](5'-DEOXYADENOSIN-5'-YL)(METHYL)SULFONIUM'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of 1-aminocyclopropane-1-carboxylate (ACC) synthase | The crystal structure of 1-aminocyclopropane-1-carboxylate (ACC) synthase in complex with the substrate analogue [2-(amino-oxy)ethyl](5'-deoxyadenosin-5'-yl)(methyl)sulfonium (AMA) was determined at 2.01-A resolution. The crystallographic results show that a covalent adduct (oxime) is formed between AMA (an amino-oxy analogue of the natural substrate S-adenosyl-L-methionine (SAM)) and the pyridoxal 5'-phosphate (PLP) cofactor of ACC synthase. The oxime formation is supported by spectroscopic data. The ACC synthase-AMA structure provides reliable and detailed information on the binding mode of the natural substrate of ACC synthase and complements previous structural and functional work on this enzyme. | ||
==About this Structure== | ==About this Structure== | ||
1M4N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Malus_x_domestica Malus x domestica] with PLP, AAD and MES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/1-aminocyclopropane-1-carboxylate_synthase 1-aminocyclopropane-1-carboxylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.14 4.4.1.14] Full crystallographic information is available from [http:// | 1M4N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Malus_x_domestica Malus x domestica] with <scene name='pdbligand=PLP:'>PLP</scene>, <scene name='pdbligand=AAD:'>AAD</scene> and <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/1-aminocyclopropane-1-carboxylate_synthase 1-aminocyclopropane-1-carboxylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.14 4.4.1.14] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4N OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Capitani, G.]] | [[Category: Capitani, G.]] | ||
[[Category: Eliot, A | [[Category: Eliot, A C.]] | ||
[[Category: Grutter, M | [[Category: Grutter, M G.]] | ||
[[Category: Gut, H.]] | [[Category: Gut, H.]] | ||
[[Category: Khomutov, R | [[Category: Khomutov, R M.]] | ||
[[Category: Kirsch, J | [[Category: Kirsch, J F.]] | ||
[[Category: AAD]] | [[Category: AAD]] | ||
[[Category: MES]] | [[Category: MES]] | ||
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[[Category: pyridoxal phosphate]] | [[Category: pyridoxal phosphate]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:24 2008'' | ||
