1miv: Difference between revisions

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[[Image:1miv.gif|left|200px]]<br /><applet load="1miv" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1miv.gif|left|200px]]<br /><applet load="1miv" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1miv, resolution 3.50Å" />

'''Crystal structure of Bacillus stearothermophilus CCA-adding enzyme'''<br />

==Overview==

CCA-adding enzymes polymerize CCA onto the 3' terminus of immature tRNAs, without using a nucleic acid template. The 3.0 A resolution crystal, structures of the CCA-adding enzyme from Bacillus stearothermophilus and, its complexes with ATP or CTP reveal a seahorse-shaped subunit consisting, of four domains: head, neck, body, and tail. The head is structurally, homologous to the palm domain of DNA polymerase beta but has additional, structural features and functions. The neck, body, and tail represent new, protein folding motifs. The neck provides a specific template for the, incoming ATP or CTP, whereas the body and tail may bind tRNA. Each subunit, has one active site capable of switching its base specificity between ATP, and CTP, an important component of the CCA-adding mechanism.

CCA-adding enzymes polymerize CCA onto the 3' terminus of immature tRNAs without using a nucleic acid template. The 3.0 A resolution crystal structures of the CCA-adding enzyme from Bacillus stearothermophilus and its complexes with ATP or CTP reveal a seahorse-shaped subunit consisting of four domains: head, neck, body, and tail. The head is structurally homologous to the palm domain of DNA polymerase beta but has additional structural features and functions. The neck, body, and tail represent new protein folding motifs. The neck provides a specific template for the incoming ATP or CTP, whereas the body and tail may bind tRNA. Each subunit has one active site capable of switching its base specificity between ATP and CTP, an important component of the CCA-adding mechanism.

==About this Structure==

1MIV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1MIV OCA].

1MIV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIV OCA].

==Reference==

Line 13:

[[Category: Geobacillus stearothermophilus]]

[[Category: Single protein]]

[[Category: Cho, H.D.]]

[[Category: Cho, H D.]]

[[Category: Li, F.]]

[[Category: Steitz, T.A.]]

[[Category: Steitz, T A.]]

[[Category: Wang, J.]]

[[Category: Weiner, A.M.]]

[[Category: Weiner, A M.]]

[[Category: Xiong, Y.]]

[[Category: MG]]

Line 23:

[[Category: trna nucleotidyltransferase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 21:26:~~33 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:37 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1miv.gif|left|200px]]<br /><applet load="1miv" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1miv.gif|left|200px]]<br /><applet load="1miv" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1miv, resolution 3.50&Aring;" />
 '''Crystal structure of Bacillus stearothermophilus CCA-adding enzyme'''<br />
 ==Overview==
-CCA-adding enzymes polymerize CCA onto the 3' terminus of immature tRNAs, without using a nucleic acid template. The 3.0 A resolution crystal, structures of the CCA-adding enzyme from Bacillus stearothermophilus and, its complexes with ATP or CTP reveal a seahorse-shaped subunit consisting, of four domains: head, neck, body, and tail. The head is structurally, homologous to the palm domain of DNA polymerase beta but has additional, structural features and functions. The neck, body, and tail represent new, protein folding motifs. The neck provides a specific template for the, incoming ATP or CTP, whereas the body and tail may bind tRNA. Each subunit, has one active site capable of switching its base specificity between ATP, and CTP, an important component of the CCA-adding mechanism.
+CCA-adding enzymes polymerize CCA onto the 3' terminus of immature tRNAs without using a nucleic acid template. The 3.0 A resolution crystal structures of the CCA-adding enzyme from Bacillus stearothermophilus and its complexes with ATP or CTP reveal a seahorse-shaped subunit consisting of four domains: head, neck, body, and tail. The head is structurally homologous to the palm domain of DNA polymerase beta but has additional structural features and functions. The neck, body, and tail represent new protein folding motifs. The neck provides a specific template for the incoming ATP or CTP, whereas the body and tail may bind tRNA. Each subunit has one active site capable of switching its base specificity between ATP and CTP, an important component of the CCA-adding mechanism.
 ==About this Structure==
-MIV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MIV OCA].
+MIV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIV OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Geobacillus stearothermophilus]]
 [[Category: Single protein]]
-[[Category: Cho, H.D.]]
+[[Category: Cho, H D.]]
 [[Category: Li, F.]]
-[[Category: Steitz, T.A.]]
+[[Category: Steitz, T A.]]
 [[Category: Wang, J.]]
-[[Category: Weiner, A.M.]]
+[[Category: Weiner, A M.]]
 [[Category: Xiong, Y.]]
 [[Category: MG]]
@@ Line 23: / Line 23: @@
 [[Category: trna nucleotidyltransferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:26:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:37 2008''