1mjg: Difference between revisions
New page: left|200px<br /><applet load="1mjg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mjg, resolution 2.2Å" /> '''CRYSTAL STRUCTURE OF ... |
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[[Image:1mjg.gif|left|200px]]<br /><applet load="1mjg" size=" | [[Image:1mjg.gif|left|200px]]<br /><applet load="1mjg" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1mjg, resolution 2.2Å" /> | caption="1mjg, resolution 2.2Å" /> | ||
'''CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)'''<br /> | '''CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)'''<br /> | ||
==Overview== | ==Overview== | ||
A metallocofactor containing iron, sulfur, copper, and nickel has been | A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit. | ||
==About this Structure== | ==About this Structure== | ||
1MJG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica] with CU1, NI, ACT, SF4 and XCC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2] Full crystallographic information is available from [http:// | 1MJG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica] with <scene name='pdbligand=CU1:'>CU1</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=XCC:'>XCC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJG OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Moorella thermoacetica]] | [[Category: Moorella thermoacetica]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Doukov, T | [[Category: Doukov, T I.]] | ||
[[Category: Drennan, C | [[Category: Drennan, C L.]] | ||
[[Category: Iverson, T | [[Category: Iverson, T M.]] | ||
[[Category: Ragsdale, S | [[Category: Ragsdale, S W.]] | ||
[[Category: Seravalli, J.]] | [[Category: Seravalli, J.]] | ||
[[Category: ACT]] | [[Category: ACT]] | ||
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[[Category: wood-ljundahl pathway]] | [[Category: wood-ljundahl pathway]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:45 2008'' | ||
Revision as of 11:55, 21 February 2008
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CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)
Overview
A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit.
About this Structure
1MJG is a Protein complex structure of sequences from Moorella thermoacetica with CU1, NI, ACT, SF4 and XCC as ligands. Active as Carbon-monoxide dehydrogenase (acceptor), with EC number 1.2.99.2 Full crystallographic information is available from OCA.
Reference
A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase., Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL, Science. 2002 Oct 18;298(5593):567-72. PMID:12386327
Page seeded by OCA on Thu Feb 21 13:55:45 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Carbon-monoxide dehydrogenase (acceptor)
- Moorella thermoacetica
- Protein complex
- Doukov, T I.
- Drennan, C L.
- Iverson, T M.
- Ragsdale, S W.
- Seravalli, J.
- ACT
- CU1
- NI
- SF4
- XCC
- Acetyl-coa synthase (acs)
- Carbon monoxide dehydrogenase(codh)
- Clostridium thermoaceticum
- Electron transfer
- Helical domain
- Hydrophobic co channel
- Nickel-coopper-iron-sulfur (ni-cu-fe-s) cluster
- Nickel-iron-sulfur cluster (ni-fe-s)
- Rossmann fold
- Substrate tunnel
- Wood-ljundahl pathway
