1mlv: Difference between revisions
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New page: left|200px<br /><applet load="1mlv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mlv, resolution 2.60Å" /> '''Structure and Cataly... |
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[[Image:1mlv.gif|left|200px]]<br /><applet load="1mlv" size=" | [[Image:1mlv.gif|left|200px]]<br /><applet load="1mlv" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1mlv, resolution 2.60Å" /> | caption="1mlv, resolution 2.60Å" /> | ||
'''Structure and Catalytic Mechanism of a SET Domain Protein Methyltransferase'''<br /> | '''Structure and Catalytic Mechanism of a SET Domain Protein Methyltransferase'''<br /> | ||
==Overview== | ==Overview== | ||
Protein lysine methylation by SET domain enzymes regulates chromatin | Protein lysine methylation by SET domain enzymes regulates chromatin structure, gene silencing, transcriptional activation, plant metabolism, and other processes. The 2.6 A resolution structure of Rubisco large subunit methyltransferase in a pseudo-bisubstrate complex with S-adenosylhomocysteine and a HEPES ion reveals an all-beta architecture for the SET domain embedded within a larger alpha-helical enzyme fold. Conserved regions of the SET domain bind S-adenosylmethionine and substrate lysine at two sites connected by a pore. We propose that methyl transfer is catalyzed by a conserved Tyr at a narrow pore connecting the sites. The cofactor enters by a "back door" on the opposite side of the enzyme from substrate, promoting highly specific protein recognition and allowing addition of multiple methyl groups. | ||
==About this Structure== | ==About this Structure== | ||
1MLV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with SAH and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/[Ribulose-bisphosphate_carboxylase]-lysine_N-methyltransferase [Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.127 2.1.1.127] Full crystallographic information is available from [http:// | 1MLV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with <scene name='pdbligand=SAH:'>SAH</scene> and <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/[Ribulose-bisphosphate_carboxylase]-lysine_N-methyltransferase [Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.127 2.1.1.127] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: [Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase]] | [[Category: [Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase]] | ||
[[Category: Beach, B | [[Category: Beach, B M.]] | ||
[[Category: Dirk, L | [[Category: Dirk, L M.A.]] | ||
[[Category: Houtz, R | [[Category: Houtz, R L.]] | ||
[[Category: Hurley, J | [[Category: Hurley, J H.]] | ||
[[Category: Trievel, R | [[Category: Trievel, R C.]] | ||
[[Category: EPE]] | [[Category: EPE]] | ||
[[Category: SAH]] | [[Category: SAH]] | ||
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[[Category: set domain]] | [[Category: set domain]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:56:31 2008'' | ||
