1muq: Difference between revisions
New page: left|200px<br /><applet load="1muq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1muq, resolution 2.30Å" /> '''X-ray Crystal Struct... |
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[[Image:1muq.gif|left|200px]]<br /><applet load="1muq" size=" | [[Image:1muq.gif|left|200px]]<br /><applet load="1muq" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1muq, resolution 2.30Å" /> | caption="1muq, resolution 2.30Å" /> | ||
'''X-ray Crystal Structure of Rattlesnake Venom Complexed With Thiodigalactoside'''<br /> | '''X-ray Crystal Structure of Rattlesnake Venom Complexed With Thiodigalactoside'''<br /> | ||
==Overview== | ==Overview== | ||
Rattlesnake venom lectin (RSL) from the western diamondback rattlesnake | Rattlesnake venom lectin (RSL) from the western diamondback rattlesnake (Crotalus atrox) is an oligomeric galactose-specific C-type lectin. The X-ray crystal structure of RSL, in complex with lactose and thiodigalactoside, at 2.2 and 2.3 A resolution, respectively, reveals a decameric protein composed of two 5-fold symmetric pentamers arranged in a staggered, back-to-back orientation. Each monomer corresponds to a single canonical C-type lectin carbohydrate recognition domain devoid of accessory domains and is disulfide-bonded to a monomer in the other pentamer. The structure is the first example of that of a carbohydrate complex of a vertebrate galactose-specific C-type lectin. The 10 carbohydrate-binding sites, located on the rim of the decamer, suggest a role for multivalent interactions and a mechanism for RSL's ability to promote receptor cross-linking and cell aggregation. | ||
==About this Structure== | ==About this Structure== | ||
1MUQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Crotalus_atrox Crotalus atrox] with GAL, CA, NA and TDG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1MUQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Crotalus_atrox Crotalus atrox] with <scene name='pdbligand=GAL:'>GAL</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=TDG:'>TDG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUQ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Hirama, T.]] | [[Category: Hirama, T.]] | ||
[[Category: Nagar, B.]] | [[Category: Nagar, B.]] | ||
[[Category: Rini, J | [[Category: Rini, J M.]] | ||
[[Category: Walker, J | [[Category: Walker, J R.]] | ||
[[Category: Young, N | [[Category: Young, N M.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: GAL]] | [[Category: GAL]] | ||
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[[Category: protein-carbohydrate complex]] | [[Category: protein-carbohydrate complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:11 2008'' | ||
Revision as of 11:59, 21 February 2008
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X-ray Crystal Structure of Rattlesnake Venom Complexed With Thiodigalactoside
Overview
Rattlesnake venom lectin (RSL) from the western diamondback rattlesnake (Crotalus atrox) is an oligomeric galactose-specific C-type lectin. The X-ray crystal structure of RSL, in complex with lactose and thiodigalactoside, at 2.2 and 2.3 A resolution, respectively, reveals a decameric protein composed of two 5-fold symmetric pentamers arranged in a staggered, back-to-back orientation. Each monomer corresponds to a single canonical C-type lectin carbohydrate recognition domain devoid of accessory domains and is disulfide-bonded to a monomer in the other pentamer. The structure is the first example of that of a carbohydrate complex of a vertebrate galactose-specific C-type lectin. The 10 carbohydrate-binding sites, located on the rim of the decamer, suggest a role for multivalent interactions and a mechanism for RSL's ability to promote receptor cross-linking and cell aggregation.
About this Structure
1MUQ is a Single protein structure of sequence from Crotalus atrox with GAL, CA, NA and TDG as ligands. Full crystallographic information is available from OCA.
Reference
X-ray crystal structure of a galactose-specific C-type lectin possessing a novel decameric quaternary structure., Walker JR, Nagar B, Young NM, Hirama T, Rini JM, Biochemistry. 2004 Apr 6;43(13):3783-92. PMID:15049685
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