1mvr: Difference between revisions

Line 1:

[[Image:1mvr.gif|left|200px]]<br /><applet load="1mvr" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1mvr.gif|left|200px]]<br /><applet load="1mvr" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1mvr" />

'''Decoding Center & Peptidyl transferase center from the X-ray structure of the Thermus thermophilus 70S ribosome, aligned to the low resolution Cryo-EM map of E.coli 70S Ribosome'''<br />

==Overview==

Protein synthesis takes place on the ribosome, where genetic information, carried by messenger RNA is translated into a sequence of amino acids., This process is terminated when a stop codon moves into the ribosomal, decoding centre (DC) and is recognized by a class-1 release factor (RF)., RFs have a conserved GGQ amino-acid motif, which is crucial for peptide, release and is believed to interact directly with the peptidyl-transferase, centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs, (SPF in RF2) has been proposed to interact directly with stop codons in, the DC of the 30S subunit. The distance between the DC and PTC is, approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ, are only 23 A apart, indicating that they cannot be at DC and PTC, simultaneously. Here we show that RF2 is in an open conformation when, bound to the ribosome, allowing GGQ to reach the PTC while still allowing, SPF-stop-codon interaction. The results indicate new interpretations of, accuracy in termination, and have implications for how the presence of a, stop codon in the DC is signalled to PTC.

Protein synthesis takes place on the ribosome, where genetic information carried by messenger RNA is translated into a sequence of amino acids. This process is terminated when a stop codon moves into the ribosomal decoding centre (DC) and is recognized by a class-1 release factor (RF). RFs have a conserved GGQ amino-acid motif, which is crucial for peptide release and is believed to interact directly with the peptidyl-transferase centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs (SPF in RF2) has been proposed to interact directly with stop codons in the DC of the 30S subunit. The distance between the DC and PTC is approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ are only 23 A apart, indicating that they cannot be at DC and PTC simultaneously. Here we show that RF2 is in an open conformation when bound to the ribosome, allowing GGQ to reach the PTC while still allowing SPF-stop-codon interaction. The results indicate new interpretations of accuracy in termination, and have implications for how the presence of a stop codon in the DC is signalled to PTC.

==About this Structure==

1MVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1MVR OCA].

1MVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVR OCA].

==Reference==

Line 15:

[[Category: Ehrenberg, M.]]

[[Category: Frank, J.]]

[[Category: Grassucci, R.A.]]

[[Category: Grassucci, R A.]]

[[Category: Linde, J.]]

[[Category: Rawat, U.B.]]

[[Category: Rawat, U B.]]

[[Category: Sengupta, J.]]

[[Category: Valle, M.]]

[[Category: Vestergaard, B.]]

[[Category: Zavialov, A.V.]]

[[Category: Zavialov, A V.]]

[[Category: conformational changes]]

[[Category: release complex]]

[[Category: rf2]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 21:44:~~31 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:32 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1mvr.gif|left|200px]]<br /><applet load="1mvr" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1mvr.gif|left|200px]]<br /><applet load="1mvr" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1mvr" />
 '''Decoding Center & Peptidyl transferase center from the X-ray structure of the Thermus thermophilus 70S ribosome, aligned to the low resolution Cryo-EM map of E.coli 70S Ribosome'''<br />
 ==Overview==
-Protein synthesis takes place on the ribosome, where genetic information, carried by messenger RNA is translated into a sequence of amino acids., This process is terminated when a stop codon moves into the ribosomal, decoding centre (DC) and is recognized by a class-1 release factor (RF)., RFs have a conserved GGQ amino-acid motif, which is crucial for peptide, release and is believed to interact directly with the peptidyl-transferase, centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs, (SPF in RF2) has been proposed to interact directly with stop codons in, the DC of the 30S subunit. The distance between the DC and PTC is, approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ, are only 23 A apart, indicating that they cannot be at DC and PTC, simultaneously. Here we show that RF2 is in an open conformation when, bound to the ribosome, allowing GGQ to reach the PTC while still allowing, SPF-stop-codon interaction. The results indicate new interpretations of, accuracy in termination, and have implications for how the presence of a, stop codon in the DC is signalled to PTC.
+Protein synthesis takes place on the ribosome, where genetic information carried by messenger RNA is translated into a sequence of amino acids. This process is terminated when a stop codon moves into the ribosomal decoding centre (DC) and is recognized by a class-1 release factor (RF). RFs have a conserved GGQ amino-acid motif, which is crucial for peptide release and is believed to interact directly with the peptidyl-transferase centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs (SPF in RF2) has been proposed to interact directly with stop codons in the DC of the 30S subunit. The distance between the DC and PTC is approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ are only 23 A apart, indicating that they cannot be at DC and PTC simultaneously. Here we show that RF2 is in an open conformation when bound to the ribosome, allowing GGQ to reach the PTC while still allowing SPF-stop-codon interaction. The results indicate new interpretations of accuracy in termination, and have implications for how the presence of a stop codon in the DC is signalled to PTC.
 ==About this Structure==
-MVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MVR OCA].
+MVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVR OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Ehrenberg, M.]]
 [[Category: Frank, J.]]
-[[Category: Grassucci, R.A.]]
+[[Category: Grassucci, R A.]]
 [[Category: Linde, J.]]
-[[Category: Rawat, U.B.]]
+[[Category: Rawat, U B.]]
 [[Category: Sengupta, J.]]
 [[Category: Valle, M.]]
 [[Category: Vestergaard, B.]]
-[[Category: Zavialov, A.V.]]
+[[Category: Zavialov, A V.]]
 [[Category: conformational changes]]
 [[Category: release complex]]
 [[Category: rf2]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:44:31 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:32 2008''