1n2d: Difference between revisions

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[[Image:1n2d.gif|left|200px]]<br /><applet load="1n2d" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1n2d.gif|left|200px]]<br /><applet load="1n2d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1n2d, resolution 2.00Å" />

'''Ternary complex of MLC1P bound to IQ2 and IQ3 of Myo2p, a class V myosin'''<br />

==Overview==

Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces, cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its, interactions with these two proteins, Mlc1p plays a role in polarized, growth and cytokinesis. Mlc1p has been crystallized in complexes with four, different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4, and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the, complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple, anomalous dispersion (MAD) experiments based on selenomethionine, derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were, determined by molecular replacement using the partially refined structure, of Mlc1p-IQ2,3 as a search model.

Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model.

==About this Structure==

1N2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1N2D OCA].

1N2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2D OCA].

==Reference==

Line 14:

[[Category: Saccharomyces cerevisiae]]

[[Category: Dominguez, R.]]

[[Category: Lu, R.C.]]

[[Category: Lu, R C.]]

[[Category: Stafford, W.F.]]

[[Category: Stafford, W F.]]

[[Category: Terrak, M.]]

[[Category: Wu, G.]]

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[[Category: protein-peptide complex]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:30 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1n2d.gif|left|200px]]<br /><applet load="1n2d" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1n2d.gif|left|200px]]<br /><applet load="1n2d" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1n2d, resolution 2.00&Aring;" />
 '''Ternary complex of MLC1P bound to IQ2 and IQ3 of Myo2p, a class V myosin'''<br />
 ==Overview==
-Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces, cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its, interactions with these two proteins, Mlc1p plays a role in polarized, growth and cytokinesis. Mlc1p has been crystallized in complexes with four, different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4, and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the, complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple, anomalous dispersion (MAD) experiments based on selenomethionine, derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were, determined by molecular replacement using the partially refined structure, of Mlc1p-IQ2,3 as a search model.
+Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model.
 ==About this Structure==
-N2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N2D OCA].
+N2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2D OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Dominguez, R.]]
-[[Category: Lu, R.C.]]
+[[Category: Lu, R C.]]
-[[Category: Stafford, W.F.]]
+[[Category: Stafford, W F.]]
 [[Category: Terrak, M.]]
 [[Category: Wu, G.]]
@@ Line 22: / Line 22: @@
 [[Category: protein-peptide complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:53:25 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:30 2008''