1n8j: Difference between revisions

Line 1:

[[Image:1n8j.gif|left|200px]]<br /><applet load="1n8j" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1n8j.gif|left|200px]]<br /><applet load="1n8j" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1n8j, resolution 2.17Å" />

'''Crystal Structure of AhpC with Active Site Cysteine mutated to Serine (C46S)'''<br />

==Overview==

Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal, transduction. We show that bacterial 2-Cys Prxs are much less sensitive to, oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two, sequence motifs unique to the sensitive 2-Cys Prxs and comparing the, crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with, other Prx structures, we define the structural origins of sensitivity. We, suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping, resting levels of hydrogen peroxide low, while permitting higher levels, during signal transduction.

Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction. We show that bacterial 2-Cys Prxs are much less sensitive to oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two sequence motifs unique to the sensitive 2-Cys Prxs and comparing the crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with other Prx structures, we define the structural origins of sensitivity. We suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping resting levels of hydrogen peroxide low, while permitting higher levels during signal transduction.

==About this Structure==

1N8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1N8J OCA].

1N8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8J OCA].

==Reference==

Line 13:

[[Category: Salmonella typhimurium]]

[[Category: Single protein]]

[[Category: Karplus, P.A.]]

[[Category: Karplus, P A.]]

[[Category: Poole, L.B.]]

[[Category: Poole, L B.]]

[[Category: Wood, Z.A.]]

[[Category: Wood, Z A.]]

[[Category: ahpc]]

[[Category: ahpf]]

Line 24:

[[Category: peroxiredoxin]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:23 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1n8j.gif|left|200px]]<br /><applet load="1n8j" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1n8j.gif|left|200px]]<br /><applet load="1n8j" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1n8j, resolution 2.17&Aring;" />
 '''Crystal Structure of AhpC with Active Site Cysteine mutated to Serine (C46S)'''<br />
 ==Overview==
-Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal, transduction. We show that bacterial 2-Cys Prxs are much less sensitive to, oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two, sequence motifs unique to the sensitive 2-Cys Prxs and comparing the, crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with, other Prx structures, we define the structural origins of sensitivity. We, suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping, resting levels of hydrogen peroxide low, while permitting higher levels, during signal transduction.
+Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction. We show that bacterial 2-Cys Prxs are much less sensitive to oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two sequence motifs unique to the sensitive 2-Cys Prxs and comparing the crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with other Prx structures, we define the structural origins of sensitivity. We suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping resting levels of hydrogen peroxide low, while permitting higher levels during signal transduction.
 ==About this Structure==
-N8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N8J OCA].
+N8J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8J OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Salmonella typhimurium]]
 [[Category: Single protein]]
-[[Category: Karplus, P.A.]]
+[[Category: Karplus, P A.]]
-[[Category: Poole, L.B.]]
+[[Category: Poole, L B.]]
-[[Category: Wood, Z.A.]]
+[[Category: Wood, Z A.]]
 [[Category: ahpc]]
 [[Category: ahpf]]
@@ Line 24: / Line 24: @@
 [[Category: peroxiredoxin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:02:45 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:23 2008''