1nb6: Difference between revisions
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New page: left|200px<br /><applet load="1nb6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nb6, resolution 2.6Å" /> '''HC-J4 RNA polymerase ... |
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[[Image:1nb6.jpg|left|200px]]<br /><applet load="1nb6" size=" | [[Image:1nb6.jpg|left|200px]]<br /><applet load="1nb6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1nb6, resolution 2.6Å" /> | caption="1nb6, resolution 2.6Å" /> | ||
'''HC-J4 RNA polymerase complexed with UTP'''<br /> | '''HC-J4 RNA polymerase complexed with UTP'''<br /> | ||
==Overview== | ==Overview== | ||
Several crystal structures of the hepatitis C virus NS5B protein | Several crystal structures of the hepatitis C virus NS5B protein (genotype-1b, strain J4) complexed with metal ions, single-stranded RNA or nucleoside-triphosphates have been determined. These complexes illustrate how conserved amino acid side-chains, together with essential structural features within the active site, control nucleotide binding and likely mediate de-novo initiation. The incoming nucleotide interacts with several basic residues from an extension on the NS5B fingers domain, a beta-hairpin from the NS5B thumb domain and the C-terminal arm. The modular, bi-partite fingers domain carries a long binding groove which guides the template towards the catalytic site. The apo-polymerase structure provides unprecedented insights into potential non-nucleoside inhibitor binding sites located between palm and thumb near motif E, which is unique to RNA polymerases and reverse transcriptases. | ||
==About this Structure== | ==About this Structure== | ||
1NB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] with MN and UTP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http:// | 1NB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=UTP:'>UTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NB6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: RNA-directed RNA polymerase]] | [[Category: RNA-directed RNA polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Farrell, D | [[Category: Farrell, D J.O.]] | ||
[[Category: Jaeger, J.]] | [[Category: Jaeger, J.]] | ||
[[Category: Rowlands, D | [[Category: Rowlands, D J.]] | ||
[[Category: Trowbridge, R.]] | [[Category: Trowbridge, R.]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:11 2008'' | ||
