1nco: Difference between revisions
New page: left|200px<br /><applet load="1nco" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nco, resolution 1.8Å" /> '''STRUCTURE OF THE ANTI... |
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[[Image:1nco.gif|left|200px]]<br /><applet load="1nco" size=" | [[Image:1nco.gif|left|200px]]<br /><applet load="1nco" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1nco, resolution 1.8Å" /> | caption="1nco, resolution 1.8Å" /> | ||
'''STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN'''<br /> | '''STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN'''<br /> | ||
==Overview== | ==Overview== | ||
Structures of the protein-chromophore complex and the apoprotein form of | Structures of the protein-chromophore complex and the apoprotein form of neocarzinostatin were determined at 1.8 angstrom resolution. Neocarzinostatin is composed of a labile chromophore with DNA-cleaving activity and a stabilizing protein. The chromophore displays marked nonlinearity of the triple bonds and is bound noncovalently in a pocket formed by the two protein domains. The chromophore pi-face interacts with the phenyl ring edges of Phe52 and Phe78. The amino sugar and carbonate groups of the chromophore are solvent exposed, whereas the epoxide, acetylene groups, and carbon C-12, the site of nucleophilic thiol addition during chromophore activation, are unexposed. The position of the amino group of the chromophore carbohydrate relative to C-12 supports the idea that the amino group plays a role in thiol activation. | ||
==About this Structure== | ==About this Structure== | ||
1NCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus] with CHR and MRD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1NCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus] with <scene name='pdbligand=CHR:'>CHR</scene> and <scene name='pdbligand=MRD:'>MRD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NCO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces carzinostaticus]] | [[Category: Streptomyces carzinostaticus]] | ||
[[Category: Kim, K | [[Category: Kim, K H.]] | ||
[[Category: Kwon, B | [[Category: Kwon, B M.]] | ||
[[Category: Myers, A | [[Category: Myers, A G.]] | ||
[[Category: Rees, D | [[Category: Rees, D C.]] | ||
[[Category: CHR]] | [[Category: CHR]] | ||
[[Category: MRD]] | [[Category: MRD]] | ||
[[Category: antibacterial and antitumor protein]] | [[Category: antibacterial and antitumor protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:43 2008'' | ||
Revision as of 12:04, 21 February 2008
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STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN
Overview
Structures of the protein-chromophore complex and the apoprotein form of neocarzinostatin were determined at 1.8 angstrom resolution. Neocarzinostatin is composed of a labile chromophore with DNA-cleaving activity and a stabilizing protein. The chromophore displays marked nonlinearity of the triple bonds and is bound noncovalently in a pocket formed by the two protein domains. The chromophore pi-face interacts with the phenyl ring edges of Phe52 and Phe78. The amino sugar and carbonate groups of the chromophore are solvent exposed, whereas the epoxide, acetylene groups, and carbon C-12, the site of nucleophilic thiol addition during chromophore activation, are unexposed. The position of the amino group of the chromophore carbohydrate relative to C-12 supports the idea that the amino group plays a role in thiol activation.
About this Structure
1NCO is a Single protein structure of sequence from Streptomyces carzinostaticus with CHR and MRD as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex., Kim KH, Kwon BM, Myers AG, Rees DC, Science. 1993 Nov 12;262(5136):1042-6. PMID:8235619
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