1ncq: Difference between revisions

Line 1:

[[Image:1ncq.gif|left|200px]]<br /><applet load="1ncq" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ncq.gif|left|200px]]<br /><applet load="1ncq" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ncq, resolution 2.5Å" />

'''The structure of HRV14 when complexed with pleconaril, an antiviral compound'''<br />

==Overview==

Pleconaril is a broad-spectrum antirhinovirus and antienterovirus compound, that binds into a hydrophobic pocket within viral protein 1, stabilizing, the capsid and resulting in the inhibition of cell attachment and RNA, uncoating. When crystals of human rhinovirus 16 (HRV16) and HRV14 are, incubated with pleconaril, drug occupancy in the binding pocket is lower, than when pleconaril is introduced during assembly prior to, crystallization. This effect is far more marked in HRV16 than in HRV14 and, is more marked with pleconaril than with other compounds. These, observations are consistent with virus yield inhibition studies and, radiolabeled drug binding studies showing that the antiviral effect of, pleconaril against HRV16 is greater on the infectivity of progeny virions, than the parent input viruses. These data suggest that drug integration, into the binding pocket during assembly, or at some other late stage in, virus replication, may contribute to the antiviral activity of capsid, binding compounds.

Pleconaril is a broad-spectrum antirhinovirus and antienterovirus compound that binds into a hydrophobic pocket within viral protein 1, stabilizing the capsid and resulting in the inhibition of cell attachment and RNA uncoating. When crystals of human rhinovirus 16 (HRV16) and HRV14 are incubated with pleconaril, drug occupancy in the binding pocket is lower than when pleconaril is introduced during assembly prior to crystallization. This effect is far more marked in HRV16 than in HRV14 and is more marked with pleconaril than with other compounds. These observations are consistent with virus yield inhibition studies and radiolabeled drug binding studies showing that the antiviral effect of pleconaril against HRV16 is greater on the infectivity of progeny virions than the parent input viruses. These data suggest that drug integration into the binding pocket during assembly, or at some other late stage in virus replication, may contribute to the antiviral activity of capsid binding compounds.

==About this Structure==

1NCQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_rhinovirus_10 Human rhinovirus 10] with W11 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1NCQ OCA].

1NCQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_rhinovirus_10 Human rhinovirus 10] with <scene name='pdbligand=W11:'>W11</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NCQ OCA].

==Reference==

Line 13:

[[Category: Human rhinovirus 10]]

[[Category: Protein complex]]

[[Category: Bator, C.M.]]

[[Category: Bator, C M.]]

[[Category: Chakravarty, S.]]

[[Category: Diana, G.]]

[[Category: Pevear, D.C.]]

[[Category: Pevear, D C.]]

[[Category: Rossmann, M.G.]]

[[Category: Rossmann, M G.]]

[[Category: Simpson, A.A.]]

[[Category: Simpson, A A.]]

[[Category: Skochko, G.A.]]

[[Category: Skochko, G A.]]

[[Category: Tull, T.M.]]

[[Category: Tull, T M.]]

[[Category: Zhang, Y.]]

[[Category: W11]]

Line 28:

[[Category: rhinovirus 14]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 22~~:08:~~31 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:39 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ncq.gif|left|200px]]<br /><applet load="1ncq" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ncq.gif|left|200px]]<br /><applet load="1ncq" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ncq, resolution 2.5&Aring;" />
 '''The structure of HRV14 when complexed with pleconaril, an antiviral compound'''<br />
 ==Overview==
-Pleconaril is a broad-spectrum antirhinovirus and antienterovirus compound, that binds into a hydrophobic pocket within viral protein 1, stabilizing, the capsid and resulting in the inhibition of cell attachment and RNA, uncoating. When crystals of human rhinovirus 16 (HRV16) and HRV14 are, incubated with pleconaril, drug occupancy in the binding pocket is lower, than when pleconaril is introduced during assembly prior to, crystallization. This effect is far more marked in HRV16 than in HRV14 and, is more marked with pleconaril than with other compounds. These, observations are consistent with virus yield inhibition studies and, radiolabeled drug binding studies showing that the antiviral effect of, pleconaril against HRV16 is greater on the infectivity of progeny virions, than the parent input viruses. These data suggest that drug integration, into the binding pocket during assembly, or at some other late stage in, virus replication, may contribute to the antiviral activity of capsid, binding compounds.
+Pleconaril is a broad-spectrum antirhinovirus and antienterovirus compound that binds into a hydrophobic pocket within viral protein 1, stabilizing the capsid and resulting in the inhibition of cell attachment and RNA uncoating. When crystals of human rhinovirus 16 (HRV16) and HRV14 are incubated with pleconaril, drug occupancy in the binding pocket is lower than when pleconaril is introduced during assembly prior to crystallization. This effect is far more marked in HRV16 than in HRV14 and is more marked with pleconaril than with other compounds. These observations are consistent with virus yield inhibition studies and radiolabeled drug binding studies showing that the antiviral effect of pleconaril against HRV16 is greater on the infectivity of progeny virions than the parent input viruses. These data suggest that drug integration into the binding pocket during assembly, or at some other late stage in virus replication, may contribute to the antiviral activity of capsid binding compounds.
 ==About this Structure==
-NCQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_rhinovirus_10 Human rhinovirus 10] with W11 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NCQ OCA].
+NCQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_rhinovirus_10 Human rhinovirus 10] with <scene name='pdbligand=W11:'>W11</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NCQ OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Human rhinovirus 10]]
 [[Category: Protein complex]]
-[[Category: Bator, C.M.]]
+[[Category: Bator, C M.]]
 [[Category: Chakravarty, S.]]
 [[Category: Diana, G.]]
-[[Category: Pevear, D.C.]]
+[[Category: Pevear, D C.]]
-[[Category: Rossmann, M.G.]]
+[[Category: Rossmann, M G.]]
-[[Category: Simpson, A.A.]]
+[[Category: Simpson, A A.]]
-[[Category: Skochko, G.A.]]
+[[Category: Skochko, G A.]]
-[[Category: Tull, T.M.]]
+[[Category: Tull, T M.]]
 [[Category: Zhang, Y.]]
 [[Category: W11]]
@@ Line 28: / Line 28: @@
 [[Category: rhinovirus 14]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:08:31 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:39 2008''