1nf4: Difference between revisions

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[[Image:1nf4.gif|left|200px]]<br /><applet load="1nf4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1nf4.gif|left|200px]]<br /><applet load="1nf4" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1nf4, resolution 2.05Å" />

'''X-Ray Structure of the Desulfovibrio desulfuricans bacterioferritin: the diiron site in different states (reduced structure)'''<br />

==Overview==

The first crystal structure of a native di-iron center in an iron-storage, protein (bacterio)ferritin is reported. The protein, isolated from the, anaerobic bacterium Desulfovibrio desulfuricans, has the unique property, of having Fe-coproporphyrin III as its heme cofactor. The, three-dimensional structure of this bacterioferritin was determined in, three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of, the di-iron ferroxidase site. Conformational changes associated with these, intermediates support the idea of a route for iron entry into the protein, shell through a pore that passes through the di-iron center. Molecular, surface and electrostatic potential calculations also suggest the presence, of another ion channel, distant from the channels at the three- and, four-fold axes proposed as points of entry for the iron atoms.

The first crystal structure of a native di-iron center in an iron-storage protein (bacterio)ferritin is reported. The protein, isolated from the anaerobic bacterium Desulfovibrio desulfuricans, has the unique property of having Fe-coproporphyrin III as its heme cofactor. The three-dimensional structure of this bacterioferritin was determined in three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of the di-iron ferroxidase site. Conformational changes associated with these intermediates support the idea of a route for iron entry into the protein shell through a pore that passes through the di-iron center. Molecular surface and electrostatic potential calculations also suggest the presence of another ion channel, distant from the channels at the three- and four-fold axes proposed as points of entry for the iron atoms.

==About this Structure==

1NF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with FE2, SO4 and FEC as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1NF4 OCA].

1NF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with <scene name='pdbligand=FE2:'>FE2</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=FEC:'>FEC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF4 OCA].

==Reference==

Line 13:

[[Category: Desulfovibrio desulfuricans]]

[[Category: Single protein]]

[[Category: Carrondo, M.A.]]

[[Category: Carrondo, M A.]]

[[Category: LeGall, J.]]

[[Category: Lindley, P.]]

[[Category: Liu, M.Y.]]

[[Category: Liu, M Y.]]

[[Category: Macedo, S.]]

[[Category: Matias, P.M.]]

[[Category: Matias, P M.]]

[[Category: Mitchell, E.]]

[[Category: Romao, C.V.]]

[[Category: Romao, C V.]]

[[Category: Teixeira, M.]]

[[Category: Xavier, A.V.]]

[[Category: Xavier, A V.]]

[[Category: FE2]]

[[Category: FEC]]

Line 31:

[[Category: fe-coproporphyrin iii haem cofactor]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 22~~:12:~~27 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:25 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1nf4.gif|left|200px]]<br /><applet load="1nf4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1nf4.gif|left|200px]]<br /><applet load="1nf4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1nf4, resolution 2.05&Aring;" />
 '''X-Ray Structure of the Desulfovibrio desulfuricans bacterioferritin: the diiron site in different states (reduced structure)'''<br />
 ==Overview==
-The first crystal structure of a native di-iron center in an iron-storage, protein (bacterio)ferritin is reported. The protein, isolated from the, anaerobic bacterium Desulfovibrio desulfuricans, has the unique property, of having Fe-coproporphyrin III as its heme cofactor. The, three-dimensional structure of this bacterioferritin was determined in, three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of, the di-iron ferroxidase site. Conformational changes associated with these, intermediates support the idea of a route for iron entry into the protein, shell through a pore that passes through the di-iron center. Molecular, surface and electrostatic potential calculations also suggest the presence, of another ion channel, distant from the channels at the three- and, four-fold axes proposed as points of entry for the iron atoms.
+The first crystal structure of a native di-iron center in an iron-storage protein (bacterio)ferritin is reported. The protein, isolated from the anaerobic bacterium Desulfovibrio desulfuricans, has the unique property of having Fe-coproporphyrin III as its heme cofactor. The three-dimensional structure of this bacterioferritin was determined in three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of the di-iron ferroxidase site. Conformational changes associated with these intermediates support the idea of a route for iron entry into the protein shell through a pore that passes through the di-iron center. Molecular surface and electrostatic potential calculations also suggest the presence of another ion channel, distant from the channels at the three- and four-fold axes proposed as points of entry for the iron atoms.
 ==About this Structure==
-NF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with FE2, SO4 and FEC as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NF4 OCA].
+NF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with <scene name='pdbligand=FE2:'>FE2</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=FEC:'>FEC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NF4 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Desulfovibrio desulfuricans]]
 [[Category: Single protein]]
-[[Category: Carrondo, M.A.]]
+[[Category: Carrondo, M A.]]
 [[Category: LeGall, J.]]
 [[Category: Lindley, P.]]
-[[Category: Liu, M.Y.]]
+[[Category: Liu, M Y.]]
 [[Category: Macedo, S.]]
-[[Category: Matias, P.M.]]
+[[Category: Matias, P M.]]
 [[Category: Mitchell, E.]]
-[[Category: Romao, C.V.]]
+[[Category: Romao, C V.]]
 [[Category: Teixeira, M.]]
-[[Category: Xavier, A.V.]]
+[[Category: Xavier, A V.]]
 [[Category: FE2]]
 [[Category: FEC]]
@@ Line 31: / Line 31: @@
 [[Category: fe-coproporphyrin iii haem cofactor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:12:27 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:25 2008''