1nfv: Difference between revisions
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New page: left|200px<br /><applet load="1nfv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nfv, resolution 1.95Å" /> '''X-ray structure of D... |
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[[Image:1nfv.gif|left|200px]]<br /><applet load="1nfv" size=" | [[Image:1nfv.gif|left|200px]]<br /><applet load="1nfv" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1nfv, resolution 1.95Å" /> | caption="1nfv, resolution 1.95Å" /> | ||
'''X-ray structure of Desulfovibrio desulfuricans bacterioferritin: the diiron centre in different catalytic states (as-isolated structure)'''<br /> | '''X-ray structure of Desulfovibrio desulfuricans bacterioferritin: the diiron centre in different catalytic states (as-isolated structure)'''<br /> | ||
==Overview== | ==Overview== | ||
The first crystal structure of a native di-iron center in an iron-storage | The first crystal structure of a native di-iron center in an iron-storage protein (bacterio)ferritin is reported. The protein, isolated from the anaerobic bacterium Desulfovibrio desulfuricans, has the unique property of having Fe-coproporphyrin III as its heme cofactor. The three-dimensional structure of this bacterioferritin was determined in three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of the di-iron ferroxidase site. Conformational changes associated with these intermediates support the idea of a route for iron entry into the protein shell through a pore that passes through the di-iron center. Molecular surface and electrostatic potential calculations also suggest the presence of another ion channel, distant from the channels at the three- and four-fold axes proposed as points of entry for the iron atoms. | ||
==About this Structure== | ==About this Structure== | ||
1NFV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with FE, SO4, 3PY, FEC and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1NFV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=3PY:'>3PY</scene>, <scene name='pdbligand=FEC:'>FEC</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NFV OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Desulfovibrio desulfuricans]] | [[Category: Desulfovibrio desulfuricans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Carrondo, M | [[Category: Carrondo, M A.]] | ||
[[Category: LeGall, J.]] | [[Category: LeGall, J.]] | ||
[[Category: Lindley, P.]] | [[Category: Lindley, P.]] | ||
[[Category: Liu, M | [[Category: Liu, M Y.]] | ||
[[Category: Macedo, S.]] | [[Category: Macedo, S.]] | ||
[[Category: Matias, P | [[Category: Matias, P M.]] | ||
[[Category: Mitchell, E.]] | [[Category: Mitchell, E.]] | ||
[[Category: Romao, C | [[Category: Romao, C V.]] | ||
[[Category: Teixeira, M.]] | [[Category: Teixeira, M.]] | ||
[[Category: Xavier, A | [[Category: Xavier, A V.]] | ||
[[Category: 3PY]] | [[Category: 3PY]] | ||
[[Category: FE]] | [[Category: FE]] | ||
| Line 33: | Line 33: | ||
[[Category: haem fe-coproporphyrin iii cofactor]] | [[Category: haem fe-coproporphyrin iii cofactor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:35 2008'' | ||
