1ng4: Difference between revisions
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New page: left|200px<br /><applet load="1ng4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ng4, resolution 2.30Å" /> '''Structure of ThiO (g... |
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[[Image:1ng4.jpg|left|200px]]<br /><applet load="1ng4" size=" | [[Image:1ng4.jpg|left|200px]]<br /><applet load="1ng4" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1ng4, resolution 2.30Å" /> | caption="1ng4, resolution 2.30Å" /> | ||
'''Structure of ThiO (glycine oxidase) from Bacillus subtilis'''<br /> | '''Structure of ThiO (glycine oxidase) from Bacillus subtilis'''<br /> | ||
==Overview== | ==Overview== | ||
The thiO gene of Bacillus subtilis encodes an FAD-dependent glycine | The thiO gene of Bacillus subtilis encodes an FAD-dependent glycine oxidase. This enzyme is a homotetramer with a monomer molecular mass of 42 kDa. In this paper, we demonstrate that ThiO is required for the biosynthesis of the thiazole moiety of thiamin pyrophosphate and describe the structure of the enzyme with N-acetylglycine bound at the active site. The closest structural relatives of ThiO are sarcosine oxidase and d-amino acid oxidase. The ThiO structure, as well as the observation that N-cyclopropylglycine is a good substrate, supports a hydride transfer mechanism for the enzyme. A mechanistic proposal for the role of ThiO in thiazole biosynthesis is also described. | ||
==About this Structure== | ==About this Structure== | ||
1NG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with PO4, FAD and PEO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1NG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=PEO:'>PEO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NG4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Augustine, A.]] | [[Category: Augustine, A.]] | ||
[[Category: Begley, T | [[Category: Begley, T P.]] | ||
[[Category: Dorrestein, P | [[Category: Dorrestein, P C.]] | ||
[[Category: Ealick, S | [[Category: Ealick, S E.]] | ||
[[Category: Park, J.]] | [[Category: Park, J.]] | ||
[[Category: Settembre, E | [[Category: Settembre, E C.]] | ||
[[Category: FAD]] | [[Category: FAD]] | ||
[[Category: PEO]] | [[Category: PEO]] | ||
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[[Category: oxidase]] | [[Category: oxidase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:40 2008'' | ||
