1njp: Difference between revisions

Line 1:

[[Image:1njp.gif|left|200px]]<br /><applet load="1njp" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1njp.gif|left|200px]]<br /><applet load="1njp" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1njp, resolution 3.50Å" />

'''The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a tRNA acceptor stem mimic (ASM)'''<br />

==Overview==

Crystal structures of tRNA mimics complexed with the large ribosomal, subunit of Deinococcus radiodurans indicate that remote interactions, determine the precise orientation of tRNA in the peptidyl-transferase, center (PTC). The PTC tolerates various orientations of puromycin, derivatives and its flexibility allows the conformational rearrangements, required for peptide-bond formation. Sparsomycin binds to A2602 and alters, the PTC conformation. H69, the intersubunit-bridge connecting the PTC and, decoding site, may also participate in tRNA placement and translocation. A, spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of, symmetry identified within the PTC suggests a unified ribosomal machinery, for peptide-bond formation, A-to-P-site translocation, and entrance of, nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the, universality of this mechanism.

Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.

==About this Structure==

1NJP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1NJP OCA].

1NJP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJP OCA].

==Reference==

Line 17:

[[Category: Bashan, A.]]

[[Category: Berisio, R.]]

[[Category: Hansen, H.A.]]

[[Category: Hansen, H A.]]

[[Category: Harms, J.M.]]

[[Category: Harms, J M.]]

[[Category: Schluenzen, F.]]

[[Category: Yonath, A.]]

Line 29:

[[Category: trna]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 22~~:19:~~30 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:49 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1njp.gif|left|200px]]<br /><applet load="1njp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1njp.gif|left|200px]]<br /><applet load="1njp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1njp, resolution 3.50&Aring;" />
 '''The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a tRNA acceptor stem mimic (ASM)'''<br />
 ==Overview==
-Crystal structures of tRNA mimics complexed with the large ribosomal, subunit of Deinococcus radiodurans indicate that remote interactions, determine the precise orientation of tRNA in the peptidyl-transferase, center (PTC). The PTC tolerates various orientations of puromycin, derivatives and its flexibility allows the conformational rearrangements, required for peptide-bond formation. Sparsomycin binds to A2602 and alters, the PTC conformation. H69, the intersubunit-bridge connecting the PTC and, decoding site, may also participate in tRNA placement and translocation. A, spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of, symmetry identified within the PTC suggests a unified ribosomal machinery, for peptide-bond formation, A-to-P-site translocation, and entrance of, nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the, universality of this mechanism.
+Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.
 ==About this Structure==
-NJP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NJP OCA].
+NJP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJP OCA].
 ==Reference==
@@ Line 17: / Line 17: @@
 [[Category: Bashan, A.]]
 [[Category: Berisio, R.]]
-[[Category: Hansen, H.A.]]
+[[Category: Hansen, H A.]]
-[[Category: Harms, J.M.]]
+[[Category: Harms, J M.]]
 [[Category: Schluenzen, F.]]
 [[Category: Yonath, A.]]
@@ Line 29: / Line 29: @@
 [[Category: trna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:19:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:49 2008''