1gr7: Difference between revisions
New page: left|200px<br /> <applet load="1gr7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gr7, resolution 1.8Å" /> '''CRYSTAL STRUCTURE OF... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1GR7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]]. | 1GR7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: CUD. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]]. | ||
==Reference== | ==Reference== | ||
| Line 24: | Line 24: | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:26:30 2007'' | ||
Revision as of 11:21, 30 October 2007
|
CRYSTAL STRUCTURE OF THE DOUBLE MUTANT CYS3SER/SER100PRO FROM PSEUDOMONAS AERUGINOSA AT 1.8 A RESOLUTION
Overview
Azurin is a cupredoxin, which functions as an electron carrier. Its fold, is dominated by a beta-sheet structure. In the present study, azurin, serves as a model system to investigate the importance of a conserved, disulphide bond for protein stability and folding/unfolding. For this, purpose, we have examined two azurin mutants, the single mutant Cys3Ser, which disrupts azurin's conserved disulphide bond, and the double mutant, Cys3Ser/Ser100Pro, which contains an additional mutation at a site distant, from the conserved disulphide. The crystal structure of the azurin double, mutant has been determined to 1.8 A resolution(2), with a crystallographic, R-factor of 17.5% (R(free)=20.8%). A comparison with the wild-type, structure reveals that structural differences are limited to the sites ... [(full description)]
About this Structure
1GR7 is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with CU as [ligand]. Structure known Active Site: CUD. Full crystallographic information is available from [OCA].
Reference
Crystal structure of the double azurin mutant Cys3Ser/Ser100Pro from Pseudomonas aeruginosa at 1.8 A resolution: its folding-unfolding energy and unfolding kinetics., Okvist M, Bonander N, Sandberg A, Karlsson BG, Krengel U, Xue Y, Sjolin L, Biochim Biophys Acta. 2002 Apr 29;1596(2):336-45. PMID:12007613
Page seeded by OCA on Tue Oct 30 13:26:30 2007
