1orc: Difference between revisions

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[[Image:1orc.gif|left|200px]]<br /><applet load="1orc" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1orc.gif|left|200px]]<br /><applet load="1orc" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1orc, resolution 1.54Å" />

'''CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]'''<br />

==Overview==

A rationally designed, genetically engineered, monomeric form of the Cro, protein from bacteriophage lambda has been crystallized and its structure, determined by isomorphous replacement and refined to a resolution of 1.54, A. The structure confirms the rationale of the design but, at the same, time, reveals 1-2 A shifts throughout the monomer structure relative to, the previously determined structure of the dimeric wild-type protein., These changes include a 1.6 A main-chain shift in part of the beta-sheet, region of the molecule relative to the alpha-helical region and a 1.1 A, shift of a buried phenylalanine within the core as well as a correlated, 2.2 A shift in a solvent-exposed beta-hairpin. The conformational, adjustments appear to reflect an inherent flexibility of the protein that, is associated with its DNA-binding function.

A rationally designed, genetically engineered, monomeric form of the Cro protein from bacteriophage lambda has been crystallized and its structure determined by isomorphous replacement and refined to a resolution of 1.54 A. The structure confirms the rationale of the design but, at the same time, reveals 1-2 A shifts throughout the monomer structure relative to the previously determined structure of the dimeric wild-type protein. These changes include a 1.6 A main-chain shift in part of the beta-sheet region of the molecule relative to the alpha-helical region and a 1.1 A shift of a buried phenylalanine within the core as well as a correlated 2.2 A shift in a solvent-exposed beta-hairpin. The conformational adjustments appear to reflect an inherent flexibility of the protein that is associated with its DNA-binding function.

==About this Structure==

1ORC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA].

1ORC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA].

==Reference==

Line 13:

[[Category: Enterobacteria phage lambda]]

[[Category: Single protein]]

[[Category: Albright, R.A.]]

[[Category: Albright, R A.]]

[[Category: Matthews, B.W.]]

[[Category: Matthews, B W.]]

[[Category: Mossing, M.C.]]

[[Category: Mossing, M C.]]

[[Category: gene regulating protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov~~ 20 ~~23:07~~:~~33 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:20:43 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1orc.gif|left|200px]]<br /><applet load="1orc" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1orc.gif|left|200px]]<br /><applet load="1orc" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1orc, resolution 1.54&Aring;" />
 '''CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]'''<br />
 ==Overview==
-A rationally designed, genetically engineered, monomeric form of the Cro, protein from bacteriophage lambda has been crystallized and its structure, determined by isomorphous replacement and refined to a resolution of 1.54, A. The structure confirms the rationale of the design but, at the same, time, reveals 1-2 A shifts throughout the monomer structure relative to, the previously determined structure of the dimeric wild-type protein., These changes include a 1.6 A main-chain shift in part of the beta-sheet, region of the molecule relative to the alpha-helical region and a 1.1 A, shift of a buried phenylalanine within the core as well as a correlated, 2.2 A shift in a solvent-exposed beta-hairpin. The conformational, adjustments appear to reflect an inherent flexibility of the protein that, is associated with its DNA-binding function.
+A rationally designed, genetically engineered, monomeric form of the Cro protein from bacteriophage lambda has been crystallized and its structure determined by isomorphous replacement and refined to a resolution of 1.54 A. The structure confirms the rationale of the design but, at the same time, reveals 1-2 A shifts throughout the monomer structure relative to the previously determined structure of the dimeric wild-type protein. These changes include a 1.6 A main-chain shift in part of the beta-sheet region of the molecule relative to the alpha-helical region and a 1.1 A shift of a buried phenylalanine within the core as well as a correlated 2.2 A shift in a solvent-exposed beta-hairpin. The conformational adjustments appear to reflect an inherent flexibility of the protein that is associated with its DNA-binding function.
 ==About this Structure==
-ORC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA].
+ORC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Enterobacteria phage lambda]]
 [[Category: Single protein]]
-[[Category: Albright, R.A.]]
+[[Category: Albright, R A.]]
-[[Category: Matthews, B.W.]]
+[[Category: Matthews, B W.]]
-[[Category: Mossing, M.C.]]
+[[Category: Mossing, M C.]]
 [[Category: gene regulating protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:07:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:20:43 2008''