1osz: Difference between revisions
New page: left|200px<br /><applet load="1osz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1osz, resolution 2.1Å" /> '''MHC CLASS I H-2KB HEA... |
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[[Image:1osz.jpg|left|200px]]<br /><applet load="1osz" size=" | [[Image:1osz.jpg|left|200px]]<br /><applet load="1osz" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1osz, resolution 2.1Å" /> | caption="1osz, resolution 2.1Å" /> | ||
'''MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN'''<br /> | '''MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
The T lineage repertoire is shaped by T cell receptor (TCR)-dependent | The T lineage repertoire is shaped by T cell receptor (TCR)-dependent positive and negative thymic selection processes. Using TCR-transgenic (N15tg) beta2-microglobulin-deficient (beta2m-/-) RAG-2(-/-) H-2(b) mice specific for the VSV8 (RGYVYQGL) octapeptide bound to Kb, we identified a single weak agonist peptide variant V4L (L4) inducing phenotypic and functional T cell maturation. The cognate VSV8 peptide, in contrast, triggers negative selection. The crystal structure of L4/Kb was determined and refined to 2.1 A for comparison with the VSV8/Kb structure at similar resolution. Aside from changes on the p4 side chain of L4 and the resulting alteration of the exposed Kb Lys-66 side chain, these two structures are essentially identical. Hence, a given TCR recognizes subtle distinctions between highly related ligands, resulting in dramatically different selection outcomes. Based on these finding and the recent structural elucidation of the N15-VSV8/Kb complex, moreover, it appears that the germ-line Valpha repertoire contributes in a significant way to positive selection. | ||
==About this Structure== | ==About this Structure== | ||
1OSZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | 1OSZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Chang, H | [[Category: Chang, H C.]] | ||
[[Category: Ghendler, Y.]] | [[Category: Ghendler, Y.]] | ||
[[Category: Kern, P.]] | [[Category: Kern, P.]] | ||
[[Category: Kim, K | [[Category: Kim, K S.]] | ||
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
[[Category: Liu, J | [[Category: Liu, J H.]] | ||
[[Category: Reinherz, E | [[Category: Reinherz, E L.]] | ||
[[Category: Teng, M | [[Category: Teng, M K.]] | ||
[[Category: Wang, J | [[Category: Wang, J H.]] | ||
[[Category: Witte, T.]] | [[Category: Witte, T.]] | ||
[[Category: complex (mhc i/peptide)]] | [[Category: complex (mhc i/peptide)]] | ||
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[[Category: transmembrane protein]] | [[Category: transmembrane protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:14 2008'' | ||
Revision as of 12:21, 21 February 2008
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MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN
Overview
The T lineage repertoire is shaped by T cell receptor (TCR)-dependent positive and negative thymic selection processes. Using TCR-transgenic (N15tg) beta2-microglobulin-deficient (beta2m-/-) RAG-2(-/-) H-2(b) mice specific for the VSV8 (RGYVYQGL) octapeptide bound to Kb, we identified a single weak agonist peptide variant V4L (L4) inducing phenotypic and functional T cell maturation. The cognate VSV8 peptide, in contrast, triggers negative selection. The crystal structure of L4/Kb was determined and refined to 2.1 A for comparison with the VSV8/Kb structure at similar resolution. Aside from changes on the p4 side chain of L4 and the resulting alteration of the exposed Kb Lys-66 side chain, these two structures are essentially identical. Hence, a given TCR recognizes subtle distinctions between highly related ligands, resulting in dramatically different selection outcomes. Based on these finding and the recent structural elucidation of the N15-VSV8/Kb complex, moreover, it appears that the germ-line Valpha repertoire contributes in a significant way to positive selection.
About this Structure
1OSZ is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands., Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL, Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10061-6. PMID:9707600
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