1ou8: Difference between revisions
New page: left|200px<br /><applet load="1ou8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ou8, resolution 1.60Å" /> '''structure of an AAA+... |
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[[Image:1ou8.jpg|left|200px]]<br /><applet load="1ou8" size=" | [[Image:1ou8.jpg|left|200px]]<br /><applet load="1ou8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1ou8, resolution 1.60Å" /> | caption="1ou8, resolution 1.60Å" /> | ||
'''structure of an AAA+ protease delivery protein in complex with a peptide degradation tag'''<br /> | '''structure of an AAA+ protease delivery protein in complex with a peptide degradation tag'''<br /> | ||
==Overview== | ==Overview== | ||
Substrate selection by AAA+ ATPases that function to unfold proteins or | Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition. | ||
==About this Structure== | ==About this Structure== | ||
1OU8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1OU8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OU8 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Haemophilus influenzae]] | [[Category: Haemophilus influenzae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Baker, T | [[Category: Baker, T A.]] | ||
[[Category: Grant, R | [[Category: Grant, R A.]] | ||
[[Category: Levchenko, I.]] | [[Category: Levchenko, I.]] | ||
[[Category: Sauer, R | [[Category: Sauer, R T.]] | ||
[[Category: Wah, D | [[Category: Wah, D A.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: homodimer]] | [[Category: homodimer]] | ||
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[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:36 2008'' | ||
Revision as of 12:21, 21 February 2008
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structure of an AAA+ protease delivery protein in complex with a peptide degradation tag
Overview
Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition.
About this Structure
1OU8 is a Single protein structure of sequence from Haemophilus influenzae with MG as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag., Levchenko I, Grant RA, Wah DA, Sauer RT, Baker TA, Mol Cell. 2003 Aug;12(2):365-72. PMID:14536076
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