1oul: Difference between revisions

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[[Image:1oul.jpg|left|200px]]<br /><applet load="1oul" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1oul.jpg|left|200px]]<br /><applet load="1oul" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1oul, resolution 2.20Å" />

'''Structure of the AAA+ protease delivery protein SspB'''<br />

==Overview==

Substrate selection by AAA+ ATPases that function to unfold proteins or, alter protein conformation is often regulated by delivery or adaptor, proteins. SspB is a protein dimer that binds to the ssrA degradation tag, and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for, degradation. Here, we describe the structure of the peptide binding domain, of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution., The ssrA peptides are bound in well-defined clefts located at the extreme, ends of the SspB homodimer. SspB contacts residues within the N-terminal, and central regions of the 11 residue ssrA tag but leaves the C-terminal, residues exposed and positioned to dock with ClpX. This structure, taken, together with biochemical analysis of SspB, suggests mechanisms by which, proteins like SspB escort substrates to AAA+ ATPases and enhance the, specificity and affinity of target recognition.

Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition.

==About this Structure==

1OUL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1OUL OCA].

1OUL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUL OCA].

==Reference==

Line 13:

[[Category: Haemophilus influenzae]]

[[Category: Single protein]]

[[Category: Baker, T.A.]]

[[Category: Baker, T A.]]

[[Category: Grant, R.A.]]

[[Category: Grant, R A.]]

[[Category: Levchenko, I.]]

[[Category: Sauer, R.T.]]

[[Category: Sauer, R T.]]

[[Category: Wah, D.A.]]

[[Category: Wah, D A.]]

[[Category: homodimer]]

[[Category: ssra peptide binding protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 23~~:12:~~44 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:49 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1oul.jpg|left|200px]]<br /><applet load="1oul" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1oul.jpg|left|200px]]<br /><applet load="1oul" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1oul, resolution 2.20&Aring;" />
 '''Structure of the AAA+ protease delivery protein SspB'''<br />
 ==Overview==
-Substrate selection by AAA+ ATPases that function to unfold proteins or, alter protein conformation is often regulated by delivery or adaptor, proteins. SspB is a protein dimer that binds to the ssrA degradation tag, and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for, degradation. Here, we describe the structure of the peptide binding domain, of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution., The ssrA peptides are bound in well-defined clefts located at the extreme, ends of the SspB homodimer. SspB contacts residues within the N-terminal, and central regions of the 11 residue ssrA tag but leaves the C-terminal, residues exposed and positioned to dock with ClpX. This structure, taken, together with biochemical analysis of SspB, suggests mechanisms by which, proteins like SspB escort substrates to AAA+ ATPases and enhance the, specificity and affinity of target recognition.
+Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition.
 ==About this Structure==
-OUL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OUL OCA].
+OUL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUL OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Haemophilus influenzae]]
 [[Category: Single protein]]
-[[Category: Baker, T.A.]]
+[[Category: Baker, T A.]]
-[[Category: Grant, R.A.]]
+[[Category: Grant, R A.]]
 [[Category: Levchenko, I.]]
-[[Category: Sauer, R.T.]]
+[[Category: Sauer, R T.]]
-[[Category: Wah, D.A.]]
+[[Category: Wah, D A.]]
 [[Category: homodimer]]
 [[Category: ssra peptide binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:12:44 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:49 2008''