1owf: Difference between revisions

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[[Image:1owf.gif|left|200px]]<br /><applet load="1owf" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1owf.gif|left|200px]]<br /><applet load="1owf" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1owf, resolution 1.95Å" />

'''Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site'''<br />

==Overview==

Integration host factor (IHF) is a DNA-bending protein that recognizes its, cognate sites through indirect readout. Previous studies have shown that, binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the, center position of a conserved TTR motif in its binding site, and that, substitution of betaGlu44 with Ala prevented IHF from discriminating, between A and T at this position. We have determined the crystal, structures and relative binding affinities for all combinations of WT-IHF, and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these, structures reveals that DNA twist plays a major role in DNA recognition by, IHF, and that this geometric parameter is dependent on the dinucleotide, step and not on the bound IHF variant.

Integration host factor (IHF) is a DNA-bending protein that recognizes its cognate sites through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the center position of a conserved TTR motif in its binding site, and that substitution of betaGlu44 with Ala prevented IHF from discriminating between A and T at this position. We have determined the crystal structures and relative binding affinities for all combinations of WT-IHF and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant.

==About this Structure==

1OWF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1OWF OCA].

1OWF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OWF OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Protein complex]]

[[Category: Gardner, J.F.]]

[[Category: Gardner, J F.]]

[[Category: Lynch, T.W.]]

[[Category: Lynch, T W.]]

[[Category: Mattis, A.N.]]

[[Category: Mattis, A N.]]

[[Category: Read, E.K.]]

[[Category: Read, E K.]]

[[Category: Rice, P.A.]]

[[Category: Rice, P A.]]

[[Category: dna bending]]

[[Category: ihf]]

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[[Category: protein-dna recognition]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 23~~:14:~~55 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:20 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1owf.gif|left|200px]]<br /><applet load="1owf" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1owf.gif|left|200px]]<br /><applet load="1owf" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1owf, resolution 1.95&Aring;" />
 '''Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site'''<br />
 ==Overview==
-Integration host factor (IHF) is a DNA-bending protein that recognizes its, cognate sites through indirect readout. Previous studies have shown that, binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the, center position of a conserved TTR motif in its binding site, and that, substitution of betaGlu44 with Ala prevented IHF from discriminating, between A and T at this position. We have determined the crystal, structures and relative binding affinities for all combinations of WT-IHF, and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these, structures reveals that DNA twist plays a major role in DNA recognition by, IHF, and that this geometric parameter is dependent on the dinucleotide, step and not on the bound IHF variant.
+Integration host factor (IHF) is a DNA-bending protein that recognizes its cognate sites through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the center position of a conserved TTR motif in its binding site, and that substitution of betaGlu44 with Ala prevented IHF from discriminating between A and T at this position. We have determined the crystal structures and relative binding affinities for all combinations of WT-IHF and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant.
 ==About this Structure==
-OWF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OWF OCA].
+OWF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OWF OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Gardner, J.F.]]
+[[Category: Gardner, J F.]]
-[[Category: Lynch, T.W.]]
+[[Category: Lynch, T W.]]
-[[Category: Mattis, A.N.]]
+[[Category: Mattis, A N.]]
-[[Category: Read, E.K.]]
+[[Category: Read, E K.]]
-[[Category: Rice, P.A.]]
+[[Category: Rice, P A.]]
 [[Category: dna bending]]
 [[Category: ihf]]
@@ Line 24: / Line 24: @@
 [[Category: protein-dna recognition]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:14:55 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:20 2008''