1p7o: Difference between revisions

Line 1:

[[Image:1p7o.jpg|left|200px]]<br /><applet load="1p7o" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1p7o.jpg|left|200px]]<br /><applet load="1p7o" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1p7o, resolution 2.30Å" />

'''Crystal structure of phospholipase A2 (MIPLA4) from Micropechis ikaheka'''<br />

==Overview==

Comparison of the crystal structures of three Micropechis ikaheka, phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit, different levels of pharmacological effects) shows that their C-terminus, (residues 110-124) is the most variable. M-Type receptor binding affinity, of the isoenzymes has also been investigated and MiPLA4 binds to the, rabbit M-type receptor with high affinity. Examination of surface charges, of the isoenzymes reveals a trend of increase in positive charges with, potency. The isoenzymes are shown to oligomerize in a, concentration-dependent manner in a semi-denaturing gel. The C-termini of, the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster, together, forming a highly exposed area. A BLAST search using the sequence, of the most potent MiPLA2 results in high similarity to Staphylococcus, aureus clotting factor A and cadherin 11. This might explain the, myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.

Comparison of the crystal structures of three Micropechis ikaheka phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit different levels of pharmacological effects) shows that their C-terminus (residues 110-124) is the most variable. M-Type receptor binding affinity of the isoenzymes has also been investigated and MiPLA4 binds to the rabbit M-type receptor with high affinity. Examination of surface charges of the isoenzymes reveals a trend of increase in positive charges with potency. The isoenzymes are shown to oligomerize in a concentration-dependent manner in a semi-denaturing gel. The C-termini of the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster together, forming a highly exposed area. A BLAST search using the sequence of the most potent MiPLA2 results in high similarity to Staphylococcus aureus clotting factor A and cadherin 11. This might explain the myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.

==About this Structure==

1P7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Micropechis_ikaheka Micropechis ikaheka]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1P7O OCA].

1P7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Micropechis_ikaheka Micropechis ikaheka]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7O OCA].

==Reference==

Line 14:

[[Category: Phospholipase A(2)]]

[[Category: Protein complex]]

[[Category: Lok, S.M.]]

[[Category: Lok, S M.]]

[[Category: Swaminathan, K.]]

[[Category: micropechis ikaheka]]

Line 20:

[[Category: phospholipase a2]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 23~~:33:~~30 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:00 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1p7o.jpg|left|200px]]<br /><applet load="1p7o" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1p7o.jpg|left|200px]]<br /><applet load="1p7o" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1p7o, resolution 2.30&Aring;" />
 '''Crystal structure of phospholipase A2 (MIPLA4) from Micropechis ikaheka'''<br />
 ==Overview==
-Comparison of the crystal structures of three Micropechis ikaheka, phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit, different levels of pharmacological effects) shows that their C-terminus, (residues 110-124) is the most variable. M-Type receptor binding affinity, of the isoenzymes has also been investigated and MiPLA4 binds to the, rabbit M-type receptor with high affinity. Examination of surface charges, of the isoenzymes reveals a trend of increase in positive charges with, potency. The isoenzymes are shown to oligomerize in a, concentration-dependent manner in a semi-denaturing gel. The C-termini of, the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster, together, forming a highly exposed area. A BLAST search using the sequence, of the most potent MiPLA2 results in high similarity to Staphylococcus, aureus clotting factor A and cadherin 11. This might explain the, myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.
+Comparison of the crystal structures of three Micropechis ikaheka phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit different levels of pharmacological effects) shows that their C-terminus (residues 110-124) is the most variable. M-Type receptor binding affinity of the isoenzymes has also been investigated and MiPLA4 binds to the rabbit M-type receptor with high affinity. Examination of surface charges of the isoenzymes reveals a trend of increase in positive charges with potency. The isoenzymes are shown to oligomerize in a concentration-dependent manner in a semi-denaturing gel. The C-termini of the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster together, forming a highly exposed area. A BLAST search using the sequence of the most potent MiPLA2 results in high similarity to Staphylococcus aureus clotting factor A and cadherin 11. This might explain the myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.
 ==About this Structure==
-P7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Micropechis_ikaheka Micropechis ikaheka]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P7O OCA].
+P7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Micropechis_ikaheka Micropechis ikaheka]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7O OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Phospholipase A(2)]]
 [[Category: Protein complex]]
-[[Category: Lok, S.M.]]
+[[Category: Lok, S M.]]
 [[Category: Swaminathan, K.]]
 [[Category: micropechis ikaheka]]
@@ Line 20: / Line 20: @@
 [[Category: phospholipase a2]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:33:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:00 2008''