1p85: Difference between revisions
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New page: left|200px<br /><applet load="1p85" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p85" /> '''Real space refined coordinates of the 50S su... |
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[[Image:1p85.gif|left|200px]]<br /><applet load="1p85" size=" | [[Image:1p85.gif|left|200px]]<br /><applet load="1p85" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1p85" /> | caption="1p85" /> | ||
'''Real space refined coordinates of the 50S subunit fitted into the low resolution cryo-EM map of the EF-G.GTP state of E. coli 70S ribosome'''<br /> | '''Real space refined coordinates of the 50S subunit fitted into the low resolution cryo-EM map of the EF-G.GTP state of E. coli 70S ribosome'''<br /> | ||
==Overview== | ==Overview== | ||
Cryo-EM density maps showing the 70S ribosome of E. coli in two different | Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation. | ||
==About this Structure== | ==About this Structure== | ||
1P85 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | 1P85 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P85 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Agrawal, R | [[Category: Agrawal, R K.]] | ||
[[Category: Chapman, M | [[Category: Chapman, M S.]] | ||
[[Category: Eswar, N.]] | [[Category: Eswar, N.]] | ||
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
[[Category: Gao, H.]] | [[Category: Gao, H.]] | ||
[[Category: Harvey, S | [[Category: Harvey, S T.]] | ||
[[Category: Korostelev, A]] | [[Category: Korostelev, A]] | ||
[[Category: Roey, P | [[Category: Roey, P Van.]] | ||
[[Category: Sali, A.]] | [[Category: Sali, A.]] | ||
[[Category: Sengupta, J.]] | [[Category: Sengupta, J.]] | ||
[[Category: Stagg, S | [[Category: Stagg, S M.]] | ||
[[Category: Valle, M.]] | [[Category: Valle, M.]] | ||
[[Category: ef-g | [[Category: ef-g gtp-bound state]] | ||
[[Category: ratchet-like movement]] | [[Category: ratchet-like movement]] | ||
[[Category: real-space refinement]] | [[Category: real-space refinement]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:09 2008'' | ||
