1pcx: Difference between revisions

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[[Image:1pcx.gif|left|200px]]<br /><applet load="1pcx" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pcx.gif|left|200px]]<br /><applet load="1pcx" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pcx, resolution 2.50Å" />

'''Crystal structure of the COPII coat subunit, Sec24, complexed with a peptide from the SNARE protein Bet1'''<br />

==Overview==

The COPII coat buds transport vesicles from the endoplasmic reticulum that, incorporate cargo and SNARE molecules. Here, we show that recognition of, the ER-Golgi SNAREs Bet1, Sed5, and Sec22 occurs through three binding, sites on the Sec23/24 subcomplex of yeast COPII. The A site binds to the, YNNSNPF motif of Sed5. The B site binds to Lxx-L/M-E sequences present in, both the Bet1 and Sed5 molecules, as well as to the DxE cargo-sorting, signal. A third, spatially distinct site binds to Sec22. COPII selects the, free v-SNARE form of Bet1 because the LxxLE sequence is sequestered in the, four-helix bundle of the v-/t-SNARE complex. COPII favors Sed5 within the, Sed5/Bos1/Sec22 t-SNARE complex because t-SNARE assembly removes, autoinhibitory contacts to expose the YNNSNPF motif. The COPII coat seems, to be a specific conductor of the fusogenic forms of these SNAREs, suggesting how vesicle fusion specificity may be programmed during, budding.

The COPII coat buds transport vesicles from the endoplasmic reticulum that incorporate cargo and SNARE molecules. Here, we show that recognition of the ER-Golgi SNAREs Bet1, Sed5, and Sec22 occurs through three binding sites on the Sec23/24 subcomplex of yeast COPII. The A site binds to the YNNSNPF motif of Sed5. The B site binds to Lxx-L/M-E sequences present in both the Bet1 and Sed5 molecules, as well as to the DxE cargo-sorting signal. A third, spatially distinct site binds to Sec22. COPII selects the free v-SNARE form of Bet1 because the LxxLE sequence is sequestered in the four-helix bundle of the v-/t-SNARE complex. COPII favors Sed5 within the Sed5/Bos1/Sec22 t-SNARE complex because t-SNARE assembly removes autoinhibitory contacts to expose the YNNSNPF motif. The COPII coat seems to be a specific conductor of the fusogenic forms of these SNAREs, suggesting how vesicle fusion specificity may be programmed during budding.

==About this Structure==

1PCX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PCX OCA].

1PCX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PCX OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Saccharomyces cerevisiae]]

[[Category: Bickford, L.C.]]

[[Category: Bickford, L C.]]

[[Category: Goldberg, J.]]

[[Category: Mossessova, E.]]

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[[Category: transport protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 23~~:41:~~57 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:31 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1pcx.gif|left|200px]]<br /><applet load="1pcx" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pcx.gif|left|200px]]<br /><applet load="1pcx" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pcx, resolution 2.50&Aring;" />
 '''Crystal structure of the COPII coat subunit, Sec24, complexed with a peptide from the SNARE protein Bet1'''<br />
 ==Overview==
-The COPII coat buds transport vesicles from the endoplasmic reticulum that, incorporate cargo and SNARE molecules. Here, we show that recognition of, the ER-Golgi SNAREs Bet1, Sed5, and Sec22 occurs through three binding, sites on the Sec23/24 subcomplex of yeast COPII. The A site binds to the, YNNSNPF motif of Sed5. The B site binds to Lxx-L/M-E sequences present in, both the Bet1 and Sed5 molecules, as well as to the DxE cargo-sorting, signal. A third, spatially distinct site binds to Sec22. COPII selects the, free v-SNARE form of Bet1 because the LxxLE sequence is sequestered in the, four-helix bundle of the v-/t-SNARE complex. COPII favors Sed5 within the, Sed5/Bos1/Sec22 t-SNARE complex because t-SNARE assembly removes, autoinhibitory contacts to expose the YNNSNPF motif. The COPII coat seems, to be a specific conductor of the fusogenic forms of these SNAREs, suggesting how vesicle fusion specificity may be programmed during, budding.
+The COPII coat buds transport vesicles from the endoplasmic reticulum that incorporate cargo and SNARE molecules. Here, we show that recognition of the ER-Golgi SNAREs Bet1, Sed5, and Sec22 occurs through three binding sites on the Sec23/24 subcomplex of yeast COPII. The A site binds to the YNNSNPF motif of Sed5. The B site binds to Lxx-L/M-E sequences present in both the Bet1 and Sed5 molecules, as well as to the DxE cargo-sorting signal. A third, spatially distinct site binds to Sec22. COPII selects the free v-SNARE form of Bet1 because the LxxLE sequence is sequestered in the four-helix bundle of the v-/t-SNARE complex. COPII favors Sed5 within the Sed5/Bos1/Sec22 t-SNARE complex because t-SNARE assembly removes autoinhibitory contacts to expose the YNNSNPF motif. The COPII coat seems to be a specific conductor of the fusogenic forms of these SNAREs, suggesting how vesicle fusion specificity may be programmed during budding.
 ==About this Structure==
-PCX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PCX OCA].
+PCX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PCX OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Bickford, L.C.]]
+[[Category: Bickford, L C.]]
 [[Category: Goldberg, J.]]
 [[Category: Mossessova, E.]]
@@ Line 19: / Line 19: @@
 [[Category: transport protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:41:57 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:31 2008''