1pdm: Difference between revisions

Line 1:

[[Image:1pdm.jpg|left|200px]]<br /><applet load="1pdm" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pdm.jpg|left|200px]]<br /><applet load="1pdm" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pdm" />

'''Fitting of gp8 structure into the cryoEM reconstruction of the bacteriophage T4 baseplate'''<br />

==Overview==

The baseplate of bacteriophage T4 is a multiprotein molecular machine that, controls host cell recognition, attachment, tail sheath contraction and, viral DNA ejection. We report here the three-dimensional structure of the, baseplate-tail tube complex determined to a resolution of 12 A by, cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like, structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the, center of the dome is a needle-like structure that was previously, identified as a cell puncturing device. We have identified the locations, of six proteins with known atomic structures, and established the position, and shape of several other baseplate proteins. The baseplate structure, suggests a mechanism of baseplate triggering and structural transition, during the initial stages of T4 infection.

The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.

==About this Structure==

1PDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PDM OCA].

1PDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDM OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Arisaka, F.]]

[[Category: Chipman, P.R.]]

[[Category: Chipman, P R.]]

[[Category: Kanamaru, S.]]

[[Category: Kostyuchenko, V.A.]]

[[Category: Kostyuchenko, V A.]]

[[Category: Leiman, P.G.]]

[[Category: Leiman, P G.]]

[[Category: Mesyanzhinov, V.V.]]

[[Category: Mesyanzhinov, V V.]]

[[Category: Raaij, M.J.van.]]

[[Category: Raaij, M J.van.]]

[[Category: Rossmann, M.G.]]

[[Category: Rossmann, M G.]]

[[Category: structural protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 23~~:43:~~00 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:40 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1pdm.jpg|left|200px]]<br /><applet load="1pdm" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pdm.jpg|left|200px]]<br /><applet load="1pdm" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pdm" />
 '''Fitting of gp8 structure into the cryoEM reconstruction of the bacteriophage T4 baseplate'''<br />
 ==Overview==
-The baseplate of bacteriophage T4 is a multiprotein molecular machine that, controls host cell recognition, attachment, tail sheath contraction and, viral DNA ejection. We report here the three-dimensional structure of the, baseplate-tail tube complex determined to a resolution of 12 A by, cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like, structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the, center of the dome is a needle-like structure that was previously, identified as a cell puncturing device. We have identified the locations, of six proteins with known atomic structures, and established the position, and shape of several other baseplate proteins. The baseplate structure, suggests a mechanism of baseplate triggering and structural transition, during the initial stages of T4 infection.
+The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.
 ==About this Structure==
-PDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PDM OCA].
+PDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDM OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Arisaka, F.]]
-[[Category: Chipman, P.R.]]
+[[Category: Chipman, P R.]]
 [[Category: Kanamaru, S.]]
-[[Category: Kostyuchenko, V.A.]]
+[[Category: Kostyuchenko, V A.]]
-[[Category: Leiman, P.G.]]
+[[Category: Leiman, P G.]]
-[[Category: Mesyanzhinov, V.V.]]
+[[Category: Mesyanzhinov, V V.]]
-[[Category: Raaij, M.J.van.]]
+[[Category: Raaij, M J.van.]]
-[[Category: Rossmann, M.G.]]
+[[Category: Rossmann, M G.]]
 [[Category: structural protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:43:00 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:40 2008''