1psp: Difference between revisions

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[[Image:1psp.gif|left|200px]]<br /><applet load="1psp" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1psp.gif|left|200px]]<br /><applet load="1psp" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1psp, resolution 2.5Å" />

'''PANCREATIC SPASMOLYTIC POLYPEPTIDE: FIRST THREE-DIMENSIONAL STRUCTURE OF A MEMBER OF THE MAMMALIAN TREFOIL FAMILY OF PEPTIDES'''<br />

==Overview==

BACKGROUND: The trefoil peptides are a rapidly growing family of peptides, mainly found in the gastrointestinal tract. There is circumstantial, evidence that they stabilize the mucus layer, and may affect the rate of, healing of the mucosal epithelium. RESULTS: We have determined the, structure of porcine pancreatic spasmolytic polypeptide (PSP) to 2.5 A, resolution. The polypeptide contains two trefoil domains. The domain, structure is compact, and is composed of a central short antiparallel, beta-sheet with one short helix above and one below it. This is a novel, motif. The two domains are related by two-fold symmetry, and each domain, contains a cleft. CONCLUSIONS: The cleft within each domain could, accommodate a polysaccharide chain, and may therefore be responsible for, binding mucin glycoproteins. We suggest that PSP may cross-link, glycoproteins, explaining its ability to stabilize the mucus layer.

BACKGROUND: The trefoil peptides are a rapidly growing family of peptides, mainly found in the gastrointestinal tract. There is circumstantial evidence that they stabilize the mucus layer, and may affect the rate of healing of the mucosal epithelium. RESULTS: We have determined the structure of porcine pancreatic spasmolytic polypeptide (PSP) to 2.5 A resolution. The polypeptide contains two trefoil domains. The domain structure is compact, and is composed of a central short antiparallel beta-sheet with one short helix above and one below it. This is a novel motif. The two domains are related by two-fold symmetry, and each domain contains a cleft. CONCLUSIONS: The cleft within each domain could accommodate a polysaccharide chain, and may therefore be responsible for binding mucin glycoproteins. We suggest that PSP may cross-link glycoproteins, explaining its ability to stabilize the mucus layer.

==About this Structure==

1PSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PSP OCA].

1PSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PSP OCA].

==Reference==

Line 16:

[[Category: Gajhede, M.]]

[[Category: Henriksen, A.]]

[[Category: Petersen, J.F.W.]]

[[Category: Petersen, J F.W.]]

[[Category: Petersen, T.N.]]

[[Category: Petersen, T N.]]

[[Category: Thim, L.]]

[[Category: Wilson, K.S.]]

[[Category: Wilson, K S.]]

[[Category: spasmolytic protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 00:05:~~23 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:32:13 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1psp.gif|left|200px]]<br /><applet load="1psp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1psp.gif|left|200px]]<br /><applet load="1psp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1psp, resolution 2.5&Aring;" />
 '''PANCREATIC SPASMOLYTIC POLYPEPTIDE: FIRST THREE-DIMENSIONAL STRUCTURE OF A MEMBER OF THE MAMMALIAN TREFOIL FAMILY OF PEPTIDES'''<br />
 ==Overview==
-BACKGROUND: The trefoil peptides are a rapidly growing family of peptides, mainly found in the gastrointestinal tract. There is circumstantial, evidence that they stabilize the mucus layer, and may affect the rate of, healing of the mucosal epithelium. RESULTS: We have determined the, structure of porcine pancreatic spasmolytic polypeptide (PSP) to 2.5 A, resolution. The polypeptide contains two trefoil domains. The domain, structure is compact, and is composed of a central short antiparallel, beta-sheet with one short helix above and one below it. This is a novel, motif. The two domains are related by two-fold symmetry, and each domain, contains a cleft. CONCLUSIONS: The cleft within each domain could, accommodate a polysaccharide chain, and may therefore be responsible for, binding mucin glycoproteins. We suggest that PSP may cross-link, glycoproteins, explaining its ability to stabilize the mucus layer.
+BACKGROUND: The trefoil peptides are a rapidly growing family of peptides, mainly found in the gastrointestinal tract. There is circumstantial evidence that they stabilize the mucus layer, and may affect the rate of healing of the mucosal epithelium. RESULTS: We have determined the structure of porcine pancreatic spasmolytic polypeptide (PSP) to 2.5 A resolution. The polypeptide contains two trefoil domains. The domain structure is compact, and is composed of a central short antiparallel beta-sheet with one short helix above and one below it. This is a novel motif. The two domains are related by two-fold symmetry, and each domain contains a cleft. CONCLUSIONS: The cleft within each domain could accommodate a polysaccharide chain, and may therefore be responsible for binding mucin glycoproteins. We suggest that PSP may cross-link glycoproteins, explaining its ability to stabilize the mucus layer.
 ==About this Structure==
-PSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PSP OCA].
+PSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PSP OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Gajhede, M.]]
 [[Category: Henriksen, A.]]
-[[Category: Petersen, J.F.W.]]
+[[Category: Petersen, J F.W.]]
-[[Category: Petersen, T.N.]]
+[[Category: Petersen, T N.]]
 [[Category: Thim, L.]]
-[[Category: Wilson, K.S.]]
+[[Category: Wilson, K S.]]
 [[Category: spasmolytic protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:05:23 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:32:13 2008''