1pvr: Difference between revisions

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[[Image:1pvr.gif|left|200px]]<br /><applet load="1pvr" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pvr.gif|left|200px]]<br /><applet load="1pvr" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pvr, resolution 2.65Å" />

'''BASIS FOR A SWITCH IN SUBSTRATE SPECIFICITY: CRYSTAL STRUCTURE OF SELECTED VARIANT OF CRE SITE-SPECIFIC RECOMBINASE, LNSGG BOUND TO THE LOXP (WILDTYPE) RECOGNITION SITE'''<br />

==Overview==

The basis for the altered DNA specificities of two Cre recombinase, variants, obtained by mutation and selection, was revealed by their, cocrystal structures. The proteins share similar substitutions but differ, in their preferences for the natural LoxP substrate and an engineered, substrate that is inactive with wild-type Cre, LoxM7. One variant, preferentially recombines LoxM7 and contacts the substituted bases through, a hydrated network of novel interlocking protein-DNA contacts. The other, variant recognizes both LoxP and LoxM7 utilizing the same DNA backbone, contact but different base contacts, facilitated by an unexpected DNA, shift. Assisted by water, novel interaction networks can arise from few, protein substitutions, suggesting how new DNA binding specificities might, evolve. The contributions of macromolecular plasticity and water networks, in specific DNA recognition observed here present a challenge for, predictive schemes.

The basis for the altered DNA specificities of two Cre recombinase variants, obtained by mutation and selection, was revealed by their cocrystal structures. The proteins share similar substitutions but differ in their preferences for the natural LoxP substrate and an engineered substrate that is inactive with wild-type Cre, LoxM7. One variant preferentially recombines LoxM7 and contacts the substituted bases through a hydrated network of novel interlocking protein-DNA contacts. The other variant recognizes both LoxP and LoxM7 utilizing the same DNA backbone contact but different base contacts, facilitated by an unexpected DNA shift. Assisted by water, novel interaction networks can arise from few protein substitutions, suggesting how new DNA binding specificities might evolve. The contributions of macromolecular plasticity and water networks in specific DNA recognition observed here present a challenge for predictive schemes.

==About this Structure==

1PVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PVR OCA].

1PVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVR OCA].

==Reference==

Line 14:

[[Category: Protein complex]]

[[Category: Abel, J.]]

[[Category: Baldwin, E.P.]]

[[Category: Baldwin, E P.]]

[[Category: Gelato, K.A.]]

[[Category: Gelato, K A.]]

[[Category: Kim, H.]]

[[Category: Martin, S.S.]]

[[Category: Martin, S S.]]

[[Category: Santoro, S.W.]]

[[Category: Santoro, S W.]]

[[Category: Schultz, P.G.]]

[[Category: Schultz, P G.]]

[[Category: cre]]

[[Category: cre-loxp recombination]]

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[[Category: recombinase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 00:09:~~53 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:00 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1pvr.gif|left|200px]]<br /><applet load="1pvr" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pvr.gif|left|200px]]<br /><applet load="1pvr" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pvr, resolution 2.65&Aring;" />
 '''BASIS FOR A SWITCH IN SUBSTRATE SPECIFICITY: CRYSTAL STRUCTURE OF SELECTED VARIANT OF CRE SITE-SPECIFIC RECOMBINASE, LNSGG BOUND TO THE LOXP (WILDTYPE) RECOGNITION SITE'''<br />
 ==Overview==
-The basis for the altered DNA specificities of two Cre recombinase, variants, obtained by mutation and selection, was revealed by their, cocrystal structures. The proteins share similar substitutions but differ, in their preferences for the natural LoxP substrate and an engineered, substrate that is inactive with wild-type Cre, LoxM7. One variant, preferentially recombines LoxM7 and contacts the substituted bases through, a hydrated network of novel interlocking protein-DNA contacts. The other, variant recognizes both LoxP and LoxM7 utilizing the same DNA backbone, contact but different base contacts, facilitated by an unexpected DNA, shift. Assisted by water, novel interaction networks can arise from few, protein substitutions, suggesting how new DNA binding specificities might, evolve. The contributions of macromolecular plasticity and water networks, in specific DNA recognition observed here present a challenge for, predictive schemes.
+The basis for the altered DNA specificities of two Cre recombinase variants, obtained by mutation and selection, was revealed by their cocrystal structures. The proteins share similar substitutions but differ in their preferences for the natural LoxP substrate and an engineered substrate that is inactive with wild-type Cre, LoxM7. One variant preferentially recombines LoxM7 and contacts the substituted bases through a hydrated network of novel interlocking protein-DNA contacts. The other variant recognizes both LoxP and LoxM7 utilizing the same DNA backbone contact but different base contacts, facilitated by an unexpected DNA shift. Assisted by water, novel interaction networks can arise from few protein substitutions, suggesting how new DNA binding specificities might evolve. The contributions of macromolecular plasticity and water networks in specific DNA recognition observed here present a challenge for predictive schemes.
 ==About this Structure==
-PVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PVR OCA].
+PVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVR OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Abel, J.]]
-[[Category: Baldwin, E.P.]]
+[[Category: Baldwin, E P.]]
-[[Category: Gelato, K.A.]]
+[[Category: Gelato, K A.]]
 [[Category: Kim, H.]]
-[[Category: Martin, S.S.]]
+[[Category: Martin, S S.]]
-[[Category: Santoro, S.W.]]
+[[Category: Santoro, S W.]]
-[[Category: Schultz, P.G.]]
+[[Category: Schultz, P G.]]
 [[Category: cre]]
 [[Category: cre-loxp recombination]]
@@ Line 25: / Line 25: @@
 [[Category: recombinase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:09:53 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:00 2008''