1pvs: Difference between revisions

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[[Image:1pvs.gif|left|200px]]<br /><applet load="1pvs" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pvs.gif|left|200px]]<br /><applet load="1pvs" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pvs, resolution 2.40Å" />

'''3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex'''<br />

==Overview==

Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II, (AlkA) functions primarily by removing alkylation damage from duplex and, single stranded DNA. A crystal structure of AlkA was refined to 2.0 A, resolution. This structure in turn was used to refine an AlkA-hypoxanthine, (substrate) complex structure to 2.4 A resolution. The complex structure, shows hypoxanthine located in AlkA's active site stacked between residues, W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine, structures indicate that free hypoxanthine binding in the active site may, inhibit glycosylase activity.

Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity.

==About this Structure==

1PVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with 7HP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PVS OCA].

1PVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=7HP:'>7HP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVS OCA].

==Reference==

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[[Category: reaction-product complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 00:09:~~57 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:07 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1pvs.gif|left|200px]]<br /><applet load="1pvs" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pvs.gif|left|200px]]<br /><applet load="1pvs" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pvs, resolution 2.40&Aring;" />
 '''3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex'''<br />
 ==Overview==
-Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II, (AlkA) functions primarily by removing alkylation damage from duplex and, single stranded DNA. A crystal structure of AlkA was refined to 2.0 A, resolution. This structure in turn was used to refine an AlkA-hypoxanthine, (substrate) complex structure to 2.4 A resolution. The complex structure, shows hypoxanthine located in AlkA's active site stacked between residues, W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine, structures indicate that free hypoxanthine binding in the active site may, inhibit glycosylase activity.
+Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity.
 ==About this Structure==
-PVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with 7HP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PVS OCA].
+PVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=7HP:'>7HP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVS OCA].
 ==Reference==
@@ Line 22: / Line 22: @@
 [[Category: reaction-product complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:09:57 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:07 2008''