1pvu: Difference between revisions

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[[Image:1pvu.jpg|left|200px]]<br /><applet load="1pvu" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pvu.jpg|left|200px]]<br /><applet load="1pvu" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pvu, resolution 2.4Å" />

'''THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV'''<br />

==Overview==

The crystal structure of the dimeric PvuII restriction endonuclease, (R.PvuII) has been determined at a resolution of 2.4A. The protein has a, mixed alpha/beta architecture and consists of two subdomains. Despite a, lack of sequence homology, extensive structural similarities exist between, one R.PvuII subdomain and the DNA-binding subdomain of EcoRV endonuclease, (R.EcoRV); the dimerization subdomains are unrelated. Within the similar, domains, flexible segments of R.PvuII are topologically equivalent to the, DNA-binding turns of R.EcoRV; potential catalytic residues can be deduced, from the structural similarities to R.EcoRV. Conformational flexibility is, important for the interaction with DNA. A possible classification of, endonuclease structures on the basis of the positions of the scissile, phosphates is discussed.

The crystal structure of the dimeric PvuII restriction endonuclease (R.PvuII) has been determined at a resolution of 2.4A. The protein has a mixed alpha/beta architecture and consists of two subdomains. Despite a lack of sequence homology, extensive structural similarities exist between one R.PvuII subdomain and the DNA-binding subdomain of EcoRV endonuclease (R.EcoRV); the dimerization subdomains are unrelated. Within the similar domains, flexible segments of R.PvuII are topologically equivalent to the DNA-binding turns of R.EcoRV; potential catalytic residues can be deduced from the structural similarities to R.EcoRV. Conformational flexibility is important for the interaction with DNA. A possible classification of endonuclease structures on the basis of the positions of the scissile phosphates is discussed.

==About this Structure==

1PVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PVU OCA].

1PVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVU OCA].

==Reference==

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[[Category: type ii restriction endonuclease]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 00:10:~~01 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:02 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1pvu.jpg|left|200px]]<br /><applet load="1pvu" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pvu.jpg|left|200px]]<br /><applet load="1pvu" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pvu, resolution 2.4&Aring;" />
 '''THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV'''<br />
 ==Overview==
-The crystal structure of the dimeric PvuII restriction endonuclease, (R.PvuII) has been determined at a resolution of 2.4A. The protein has a, mixed alpha/beta architecture and consists of two subdomains. Despite a, lack of sequence homology, extensive structural similarities exist between, one R.PvuII subdomain and the DNA-binding subdomain of EcoRV endonuclease, (R.EcoRV); the dimerization subdomains are unrelated. Within the similar, domains, flexible segments of R.PvuII are topologically equivalent to the, DNA-binding turns of R.EcoRV; potential catalytic residues can be deduced, from the structural similarities to R.EcoRV. Conformational flexibility is, important for the interaction with DNA. A possible classification of, endonuclease structures on the basis of the positions of the scissile, phosphates is discussed.
+The crystal structure of the dimeric PvuII restriction endonuclease (R.PvuII) has been determined at a resolution of 2.4A. The protein has a mixed alpha/beta architecture and consists of two subdomains. Despite a lack of sequence homology, extensive structural similarities exist between one R.PvuII subdomain and the DNA-binding subdomain of EcoRV endonuclease (R.EcoRV); the dimerization subdomains are unrelated. Within the similar domains, flexible segments of R.PvuII are topologically equivalent to the DNA-binding turns of R.EcoRV; potential catalytic residues can be deduced from the structural similarities to R.EcoRV. Conformational flexibility is important for the interaction with DNA. A possible classification of endonuclease structures on the basis of the positions of the scissile phosphates is discussed.
 ==About this Structure==
-PVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PVU OCA].
+PVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVU OCA].
 ==Reference==
@@ Line 18: / Line 18: @@
 [[Category: type ii restriction endonuclease]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:10:01 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:02 2008''