1q07: Difference between revisions

Line 1:

[[Image:1q07.gif|left|200px]]<br /><applet load="1q07" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1q07.gif|left|200px]]<br /><applet load="1q07" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1q07, resolution 2.50Å" />

'''Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator'''<br />

==Overview==

The earliest of a series of copper efflux genes in Escherichia coli are, controlled by CueR, a member of the MerR family of transcriptional, activators. Thermodynamic calibration of CueR reveals a zeptomolar, (10(-21) molar) sensitivity to free Cu+, which is far less than one atom, per cell. Atomic details of this extraordinary sensitivity and selectivity, for +1transition-metal ions are revealed by comparing the crystal, structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried, metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding, interactions to enhance metal binding. This binding mode is rare among, metalloproteins but well suited for an ultrasensitive genetic switch.

The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.

==About this Structure==

1Q07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with AU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1Q07 OCA].

1Q07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AU:'>AU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q07 OCA].

==Reference==

Line 15:

[[Category: Changela, A.]]

[[Category: Chen, K.]]

[[Category: Halloran, T.V.O.]]

[[Category: Halloran, T V.O.]]

[[Category: Holschen, J.]]

[[Category: Mondragon, A.]]

[[Category: Outten, C.E.]]

[[Category: Outten, C E.]]

[[Category: Xue, Y.]]

[[Category: AU]]

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[[Category: merr family transcriptional regulator]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:26 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1q07.gif|left|200px]]<br /><applet load="1q07" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1q07.gif|left|200px]]<br /><applet load="1q07" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1q07, resolution 2.50&Aring;" />
 '''Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator'''<br />
 ==Overview==
-The earliest of a series of copper efflux genes in Escherichia coli are, controlled by CueR, a member of the MerR family of transcriptional, activators. Thermodynamic calibration of CueR reveals a zeptomolar, (10(-21) molar) sensitivity to free Cu+, which is far less than one atom, per cell. Atomic details of this extraordinary sensitivity and selectivity, for +1transition-metal ions are revealed by comparing the crystal, structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried, metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding, interactions to enhance metal binding. This binding mode is rare among, metalloproteins but well suited for an ultrasensitive genetic switch.
+The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.
 ==About this Structure==
-Q07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with AU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q07 OCA].
+Q07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AU:'>AU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q07 OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Changela, A.]]
 [[Category: Chen, K.]]
-[[Category: Halloran, T.V.O.]]
+[[Category: Halloran, T V.O.]]
 [[Category: Holschen, J.]]
 [[Category: Mondragon, A.]]
-[[Category: Outten, C.E.]]
+[[Category: Outten, C E.]]
 [[Category: Xue, Y.]]
 [[Category: AU]]
@@ Line 24: / Line 24: @@
 [[Category: merr family transcriptional regulator]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:16:27 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:26 2008''