1qb4: Difference between revisions

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[[Image:1qb4.gif|left|200px]]<br /><applet load="1qb4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1qb4.gif|left|200px]]<br /><applet load="1qb4" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1qb4, resolution 2.6Å" />

'''CRYSTAL STRUCTURE OF MN(2+)-BOUND PHOSPHOENOLPYRUVATE CARBOXYLASE'''<br />

==Overview==

We have determined the crystal structure of Mn2+-bound Escherichia coli, phosphoenolpyruvate carboxylase (PEPC) using X-ray diffraction at 2.6 A, resolution, and specified the location of enzyme-bound Mn2+, which is, essential for catalytic activity. The electron density map reveals that, Mn2+ is bound to the side chain oxygens of Glu-506 and Asp-543, and, located at the top of the alpha/beta barrel in PEPC. The coordination, sphere of Mn2+ observed in E. coli PEPC is similar to that of Mn2+ found, in the pyruvate kinase structure. The model study of Mn2+-bound PEPC, complexed with phosphoenolpyruvate (PEP) reveals that the side chains of, Arg-396, Arg-581 and Arg-713 could interact with PEP.

We have determined the crystal structure of Mn2+-bound Escherichia coli phosphoenolpyruvate carboxylase (PEPC) using X-ray diffraction at 2.6 A resolution, and specified the location of enzyme-bound Mn2+, which is essential for catalytic activity. The electron density map reveals that Mn2+ is bound to the side chain oxygens of Glu-506 and Asp-543, and located at the top of the alpha/beta barrel in PEPC. The coordination sphere of Mn2+ observed in E. coli PEPC is similar to that of Mn2+ found in the pyruvate kinase structure. The model study of Mn2+-bound PEPC complexed with phosphoenolpyruvate (PEP) reveals that the side chains of Arg-396, Arg-581 and Arg-713 could interact with PEP.

==About this Structure==

1QB4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN and ASP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxylase Phosphoenolpyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.31 4.1.1.31] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1QB4 OCA].

1QB4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=ASP:'>ASP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxylase Phosphoenolpyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.31 4.1.1.31] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QB4 OCA].

==Reference==

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[[Category: alpha beta barrel]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 00:32:~~57 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:37:46 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1qb4.gif|left|200px]]<br /><applet load="1qb4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qb4.gif|left|200px]]<br /><applet load="1qb4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1qb4, resolution 2.6&Aring;" />
 '''CRYSTAL STRUCTURE OF MN(2+)-BOUND PHOSPHOENOLPYRUVATE CARBOXYLASE'''<br />
 ==Overview==
-We have determined the crystal structure of Mn2+-bound Escherichia coli, phosphoenolpyruvate carboxylase (PEPC) using X-ray diffraction at 2.6 A, resolution, and specified the location of enzyme-bound Mn2+, which is, essential for catalytic activity. The electron density map reveals that, Mn2+ is bound to the side chain oxygens of Glu-506 and Asp-543, and, located at the top of the alpha/beta barrel in PEPC. The coordination, sphere of Mn2+ observed in E. coli PEPC is similar to that of Mn2+ found, in the pyruvate kinase structure. The model study of Mn2+-bound PEPC, complexed with phosphoenolpyruvate (PEP) reveals that the side chains of, Arg-396, Arg-581 and Arg-713 could interact with PEP.
+We have determined the crystal structure of Mn2+-bound Escherichia coli phosphoenolpyruvate carboxylase (PEPC) using X-ray diffraction at 2.6 A resolution, and specified the location of enzyme-bound Mn2+, which is essential for catalytic activity. The electron density map reveals that Mn2+ is bound to the side chain oxygens of Glu-506 and Asp-543, and located at the top of the alpha/beta barrel in PEPC. The coordination sphere of Mn2+ observed in E. coli PEPC is similar to that of Mn2+ found in the pyruvate kinase structure. The model study of Mn2+-bound PEPC complexed with phosphoenolpyruvate (PEP) reveals that the side chains of Arg-396, Arg-581 and Arg-713 could interact with PEP.
 ==About this Structure==
-QB4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN and ASP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxylase Phosphoenolpyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.31 4.1.1.31] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QB4 OCA].
+QB4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=ASP:'>ASP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxylase Phosphoenolpyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.31 4.1.1.31] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QB4 OCA].
 ==Reference==
@@ Line 25: / Line 25: @@
 [[Category: alpha beta barrel]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:32:57 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:37:46 2008''