1qga: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1qga" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qga, resolution 2.0Å" /> '''PEA FNR Y308W MUTANT ... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1qga.gif|left|200px]]<br /><applet load="1qga" size=" | [[Image:1qga.gif|left|200px]]<br /><applet load="1qga" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1qga, resolution 2.0Å" /> | caption="1qga, resolution 2.0Å" /> | ||
'''PEA FNR Y308W MUTANT IN COMPLEX WITH NADP+'''<br /> | '''PEA FNR Y308W MUTANT IN COMPLEX WITH NADP+'''<br /> | ||
==Overview== | ==Overview== | ||
The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyzes the production | The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyzes the production of NADPH during photosynthesis. Whereas the structures of FNRs from spinach leaf and a cyanobacterium as well as many of their homologs have been solved, none of these studies has yielded a productive geometry of the flavin-nicotinamide interaction. Here, we show that this failure occurs because nicotinamide binding to wild type FNR involves the energetically unfavorable displacement of the C-terminal Tyr side chain. We used mutants of this residue (Tyr 308) of pea FNR to obtain the structures of productive NADP+ and NADPH complexes. These structures reveal a unique NADP+ binding mode in which the nicotinamide ring is not parallel to the flavin isoalloxazine ring, but lies against it at an angle of approximately 30 degrees, with the C4 atom 3 A from the flavin N5 atom. | ||
==About this Structure== | ==About this Structure== | ||
1QGA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with SO4, FAD and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Full crystallographic information is available from [http:// | 1QGA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QGA OCA]. | ||
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aliverti, A.]] | [[Category: Aliverti, A.]] | ||
[[Category: Arakaki, A | [[Category: Arakaki, A K.]] | ||
[[Category: Calcaterra, N | [[Category: Calcaterra, N B.]] | ||
[[Category: Carrillo, N.]] | [[Category: Carrillo, N.]] | ||
[[Category: Ceccarelli, E | [[Category: Ceccarelli, E A.]] | ||
[[Category: Deng, Z.]] | [[Category: Deng, Z.]] | ||
[[Category: Karplus, P | [[Category: Karplus, P A.]] | ||
[[Category: Orellano, E | [[Category: Orellano, E G.]] | ||
[[Category: Ottado, J.]] | [[Category: Ottado, J.]] | ||
[[Category: Zanetti, G.]] | [[Category: Zanetti, G.]] | ||
| Line 32: | Line 32: | ||
[[Category: photosynthesis]] | [[Category: photosynthesis]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:11 2008'' | ||
