1qtw: Difference between revisions

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[[Image:1qtw.jpg|left|200px]]<br /><applet load="1qtw" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1qtw.jpg|left|200px]]<br /><applet load="1qtw" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1qtw, resolution 1.02Å" />

'''HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI DNA REPAIR ENZYME ENDONUCLEASE IV'''<br />

==Overview==

Endonuclease IV is the archetype for a conserved apurinic/apyrimidinic, (AP) endonuclease family that primes DNA repair synthesis by cleaving the, DNA backbone 5' of AP sites. The crystal structures of Endonuclease IV and, its AP-DNA complex at 1.02 and 1.55 A resolution reveal how an alpha8beta8, TIM barrel fold can bind dsDNA. Enzyme loops intercalate side chains at, the abasic site, compress the DNA backbone, bend the DNA approximately 90, degrees, and promote double-nucleotide flipping to sequester the, extrahelical AP site in an enzyme pocket that excludes undamaged, nucleotides. These structures suggest three Zn2+ ions directly participate, in phosphodiester bond cleavage and prompt hypotheses that, double-nucleotide flipping and sharp bending by AP endonucleases provide, exquisite damage specificity while aiding subsequent base excision repair, pathway progression.

Endonuclease IV is the archetype for a conserved apurinic/apyrimidinic (AP) endonuclease family that primes DNA repair synthesis by cleaving the DNA backbone 5' of AP sites. The crystal structures of Endonuclease IV and its AP-DNA complex at 1.02 and 1.55 A resolution reveal how an alpha8beta8 TIM barrel fold can bind dsDNA. Enzyme loops intercalate side chains at the abasic site, compress the DNA backbone, bend the DNA approximately 90 degrees, and promote double-nucleotide flipping to sequester the extrahelical AP site in an enzyme pocket that excludes undamaged nucleotides. These structures suggest three Zn2+ ions directly participate in phosphodiester bond cleavage and prompt hypotheses that double-nucleotide flipping and sharp bending by AP endonucleases provide exquisite damage specificity while aiding subsequent base excision repair pathway progression.

==About this Structure==

1QTW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_IV_(phage-T(4)-induced) Deoxyribonuclease IV (phage-T(4)-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.2 3.1.21.2] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1QTW OCA].

1QTW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_IV_(phage-T(4)-induced) Deoxyribonuclease IV (phage-T(4)-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.2 3.1.21.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QTW OCA].

==Reference==

Line 14:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Cunningham, R.P.]]

[[Category: Cunningham, R P.]]

[[Category: Guan, Y.]]

[[Category: Haas, B.J.]]

[[Category: Haas, B J.]]

[[Category: Hosfield, D.J.]]

[[Category: Hosfield, D J.]]

[[Category: Tainer, J.A.]]

[[Category: Tainer, J A.]]

[[Category: ZN]]

[[Category: dna repair enzyme]]

Line 24:

[[Category: trinuclear zn cluster]]

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@@ Line 1: / Line 1: @@
-[[Image:1qtw.jpg|left|200px]]<br /><applet load="1qtw" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qtw.jpg|left|200px]]<br /><applet load="1qtw" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1qtw, resolution 1.02&Aring;" />
 '''HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI DNA REPAIR ENZYME ENDONUCLEASE IV'''<br />
 ==Overview==
-Endonuclease IV is the archetype for a conserved apurinic/apyrimidinic, (AP) endonuclease family that primes DNA repair synthesis by cleaving the, DNA backbone 5' of AP sites. The crystal structures of Endonuclease IV and, its AP-DNA complex at 1.02 and 1.55 A resolution reveal how an alpha8beta8, TIM barrel fold can bind dsDNA. Enzyme loops intercalate side chains at, the abasic site, compress the DNA backbone, bend the DNA approximately 90, degrees, and promote double-nucleotide flipping to sequester the, extrahelical AP site in an enzyme pocket that excludes undamaged, nucleotides. These structures suggest three Zn2+ ions directly participate, in phosphodiester bond cleavage and prompt hypotheses that, double-nucleotide flipping and sharp bending by AP endonucleases provide, exquisite damage specificity while aiding subsequent base excision repair, pathway progression.
+Endonuclease IV is the archetype for a conserved apurinic/apyrimidinic (AP) endonuclease family that primes DNA repair synthesis by cleaving the DNA backbone 5' of AP sites. The crystal structures of Endonuclease IV and its AP-DNA complex at 1.02 and 1.55 A resolution reveal how an alpha8beta8 TIM barrel fold can bind dsDNA. Enzyme loops intercalate side chains at the abasic site, compress the DNA backbone, bend the DNA approximately 90 degrees, and promote double-nucleotide flipping to sequester the extrahelical AP site in an enzyme pocket that excludes undamaged nucleotides. These structures suggest three Zn2+ ions directly participate in phosphodiester bond cleavage and prompt hypotheses that double-nucleotide flipping and sharp bending by AP endonucleases provide exquisite damage specificity while aiding subsequent base excision repair pathway progression.
 ==About this Structure==
-QTW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_IV_(phage-T(4)-induced) Deoxyribonuclease IV (phage-T(4)-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.2 3.1.21.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QTW OCA].
+QTW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_IV_(phage-T(4)-induced) Deoxyribonuclease IV (phage-T(4)-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.2 3.1.21.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QTW OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Cunningham, R.P.]]
+[[Category: Cunningham, R P.]]
 [[Category: Guan, Y.]]
-[[Category: Haas, B.J.]]
+[[Category: Haas, B J.]]
-[[Category: Hosfield, D.J.]]
+[[Category: Hosfield, D J.]]
-[[Category: Tainer, J.A.]]
+[[Category: Tainer, J A.]]
 [[Category: ZN]]
 [[Category: dna repair enzyme]]
@@ Line 24: / Line 24: @@
 [[Category: trinuclear zn cluster]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:58:56 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:43:43 2008''