1r1n: Difference between revisions

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[[Image:1r1n.gif|left|200px]]<br /><applet load="1r1n" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1r1n.gif|left|200px]]<br /><applet load="1r1n" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1r1n, resolution 1.74Å" />

'''Tri-nuclear oxo-iron clusters in the ferric binding protein from N. gonorrhoeae'''<br />

==Overview==

We report a set of three 1.8-1.9 A resolution X-ray crystal structures of, Neisseria gonorrhoeae Fbp (ferric-ion binding protein): (i) open-cleft, apo-Fbp containing bound phosphate, (ii) open-cleft mono-Fe Fbp capped by, nitrilotriacetate, and (iii) open-cleft trinuclear oxo-iron Fbp, the first, structure of an iron-cluster adduct of a transferrin. The nine independent, molecules in the unit cells provide 'snapshots' of the versatile dynamic, structural roles of the conserved dityrosyl iron-binding motif, (Tyr195-Tyr196) which control the capture and, possibly, processing of, iron. These findings have implications for understanding bacterial iron, acquisition and dissimilation, and organic/mineral interfaces.

We report a set of three 1.8-1.9 A resolution X-ray crystal structures of Neisseria gonorrhoeae Fbp (ferric-ion binding protein): (i) open-cleft apo-Fbp containing bound phosphate, (ii) open-cleft mono-Fe Fbp capped by nitrilotriacetate, and (iii) open-cleft trinuclear oxo-iron Fbp, the first structure of an iron-cluster adduct of a transferrin. The nine independent molecules in the unit cells provide 'snapshots' of the versatile dynamic structural roles of the conserved dityrosyl iron-binding motif (Tyr195-Tyr196) which control the capture and, possibly, processing of iron. These findings have implications for understanding bacterial iron acquisition and dissimilation, and organic/mineral interfaces.

==About this Structure==

1R1N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae] with CNB, CN1 and CNF as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1R1N OCA].

1R1N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae] with <scene name='pdbligand=CNB:'>CNB</scene>, <scene name='pdbligand=CN1:'>CN1</scene> and <scene name='pdbligand=CNF:'>CNF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1N OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Alexeev, D.]]

[[Category: Campopiano, D.J.]]

[[Category: Campopiano, D J.]]

[[Category: Hunter, D.J.]]

[[Category: Hunter, D J.]]

[[Category: Sadler, P.J.]]

[[Category: Sadler, P J.]]

[[Category: Zhu, H.]]

[[Category: CN1]]

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[[Category: iron binding protein]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:02 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1r1n.gif|left|200px]]<br /><applet load="1r1n" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1r1n.gif|left|200px]]<br /><applet load="1r1n" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1r1n, resolution 1.74&Aring;" />
 '''Tri-nuclear oxo-iron clusters in the ferric binding protein from N. gonorrhoeae'''<br />
 ==Overview==
-We report a set of three 1.8-1.9 A resolution X-ray crystal structures of, Neisseria gonorrhoeae Fbp (ferric-ion binding protein): (i) open-cleft, apo-Fbp containing bound phosphate, (ii) open-cleft mono-Fe Fbp capped by, nitrilotriacetate, and (iii) open-cleft trinuclear oxo-iron Fbp, the first, structure of an iron-cluster adduct of a transferrin. The nine independent, molecules in the unit cells provide 'snapshots' of the versatile dynamic, structural roles of the conserved dityrosyl iron-binding motif, (Tyr195-Tyr196) which control the capture and, possibly, processing of, iron. These findings have implications for understanding bacterial iron, acquisition and dissimilation, and organic/mineral interfaces.
+We report a set of three 1.8-1.9 A resolution X-ray crystal structures of Neisseria gonorrhoeae Fbp (ferric-ion binding protein): (i) open-cleft apo-Fbp containing bound phosphate, (ii) open-cleft mono-Fe Fbp capped by nitrilotriacetate, and (iii) open-cleft trinuclear oxo-iron Fbp, the first structure of an iron-cluster adduct of a transferrin. The nine independent molecules in the unit cells provide 'snapshots' of the versatile dynamic structural roles of the conserved dityrosyl iron-binding motif (Tyr195-Tyr196) which control the capture and, possibly, processing of iron. These findings have implications for understanding bacterial iron acquisition and dissimilation, and organic/mineral interfaces.
 ==About this Structure==
-R1N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae] with CNB, CN1 and CNF as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R1N OCA].
+R1N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae] with <scene name='pdbligand=CNB:'>CNB</scene>, <scene name='pdbligand=CN1:'>CN1</scene> and <scene name='pdbligand=CNF:'>CNF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1N OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Alexeev, D.]]
-[[Category: Campopiano, D.J.]]
+[[Category: Campopiano, D J.]]
-[[Category: Hunter, D.J.]]
+[[Category: Hunter, D J.]]
-[[Category: Sadler, P.J.]]
+[[Category: Sadler, P J.]]
 [[Category: Zhu, H.]]
 [[Category: CN1]]
@@ Line 23: / Line 23: @@
 [[Category: iron binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:12:09 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:02 2008''