1ra4: Difference between revisions

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[[Image:1ra4.gif|left|200px]]<br /><applet load="1ra4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ra4.gif|left|200px]]<br /><applet load="1ra4" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ra4, resolution 1.86Å" />

'''Crystal structure of the Methanococcus jannaschii L7Ae protein'''<br />

==Overview==

Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein, that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D, sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has, been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary, conservation, functional characteristics, and structural details have been, analyzed. Comparison of the L7Ae structure to those of a number of related, proteins with diverse functions has revealed significant structural, homology which suggests that this protein fold is an ancient RNA-binding, motif. Notably, the free M. jannaschii L7Ae structure is essentially, identical to that with RNA bound, suggesting that RNA binding occurs, through an induced-fit interaction. Circular dichroism experiments show, that box C/D and C'/D' RNA motifs undergo conformational changes when, magnesium or the L7Ae protein is added, corroborating the induced-fit, model for L7Ae-box C/D RNA interactions.

Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions.

==About this Structure==

1RA4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA].

1RA4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA].

==Reference==

Line 13:

[[Category: Methanocaldococcus jannaschii]]

[[Category: Single protein]]

[[Category: Brown, B.A.]]

[[Category: Brown, B A.]]

[[Category: Maxwell, E.S.]]

[[Category: Maxwell, E S.]]

[[Category: Suryadi, J.]]

[[Category: Tran, E.J.]]

[[Category: Tran, E J.]]

[[Category: alpha-beta-alpha sandwich fold]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 01:23:~~17 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:37 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ra4.gif|left|200px]]<br /><applet load="1ra4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ra4.gif|left|200px]]<br /><applet load="1ra4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ra4, resolution 1.86&Aring;" />
 '''Crystal structure of the Methanococcus jannaschii L7Ae protein'''<br />
 ==Overview==
-Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein, that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D, sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has, been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary, conservation, functional characteristics, and structural details have been, analyzed. Comparison of the L7Ae structure to those of a number of related, proteins with diverse functions has revealed significant structural, homology which suggests that this protein fold is an ancient RNA-binding, motif. Notably, the free M. jannaschii L7Ae structure is essentially, identical to that with RNA bound, suggesting that RNA binding occurs, through an induced-fit interaction. Circular dichroism experiments show, that box C/D and C'/D' RNA motifs undergo conformational changes when, magnesium or the L7Ae protein is added, corroborating the induced-fit, model for L7Ae-box C/D RNA interactions.
+Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions.
 ==About this Structure==
-RA4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA].
+RA4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Methanocaldococcus jannaschii]]
 [[Category: Single protein]]
-[[Category: Brown, B.A.]]
+[[Category: Brown, B A.]]
-[[Category: Maxwell, E.S.]]
+[[Category: Maxwell, E S.]]
 [[Category: Suryadi, J.]]
-[[Category: Tran, E.J.]]
+[[Category: Tran, E J.]]
 [[Category: alpha-beta-alpha sandwich fold]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:23:17 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:37 2008''