1rbj: Difference between revisions

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[[Image:1rbj.gif|left|200px]]<br /><applet load="1rbj" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1rbj.gif|left|200px]]<br /><applet load="1rbj" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1rbj, resolution 2.700Å" />

'''RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))'''<br />

==Overview==

The structure of a tetragonal crystal of bovine pancreatic RNase B, complexed with d(pA)(4) was determined by molecular replacement and, difference Fourier methods. This crystal belongs to space group P4(1)2(1)2, and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists, of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include, any polysaccharide residues or solvent molecules. After refinement at 2.7, A, the R value was 0.163 with acceptable stereochemistry. The model, illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.

The structure of a tetragonal crystal of bovine pancreatic RNase B complexed with d(pA)(4) was determined by molecular replacement and difference Fourier methods. This crystal belongs to space group P4(1)2(1)2 and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include any polysaccharide residues or solvent molecules. After refinement at 2.7 A, the R value was 0.163 with acceptable stereochemistry. The model illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.

==About this Structure==

1RBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1RBJ OCA].

1RBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RBJ OCA].

==Reference==

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[[Category: Pancreatic ribonuclease]]

[[Category: Single protein]]

[[Category: Ko, T.P.]]

[[Category: Ko, T P.]]

[[Category: McPherson, A.]]

[[Category: Williams, R.]]

[[Category: protein-dna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 01:26:~~26 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:49:15 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1rbj.gif|left|200px]]<br /><applet load="1rbj" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1rbj.gif|left|200px]]<br /><applet load="1rbj" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1rbj, resolution 2.700&Aring;" />
 '''RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))'''<br />
 ==Overview==
-The structure of a tetragonal crystal of bovine pancreatic RNase B, complexed with d(pA)(4) was determined by molecular replacement and, difference Fourier methods. This crystal belongs to space group P4(1)2(1)2, and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists, of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include, any polysaccharide residues or solvent molecules. After refinement at 2.7, A, the R value was 0.163 with acceptable stereochemistry. The model, illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.
+The structure of a tetragonal crystal of bovine pancreatic RNase B complexed with d(pA)(4) was determined by molecular replacement and difference Fourier methods. This crystal belongs to space group P4(1)2(1)2 and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include any polysaccharide residues or solvent molecules. After refinement at 2.7 A, the R value was 0.163 with acceptable stereochemistry. The model illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.
 ==About this Structure==
-RBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RBJ OCA].
+RBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RBJ OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Pancreatic ribonuclease]]
 [[Category: Single protein]]
-[[Category: Ko, T.P.]]
+[[Category: Ko, T P.]]
 [[Category: McPherson, A.]]
 [[Category: Williams, R.]]
 [[Category: protein-dna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:26:26 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:49:15 2008''