1rc7: Difference between revisions

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[[Image:1rc7.gif|left|200px]]<br /><applet load="1rc7" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1rc7.gif|left|200px]]<br /><applet load="1rc7" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1rc7, resolution 2.15Å" />

'''Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution'''<br />

==Overview==

Ribonuclease III (RNase III) represents a family of double-stranded RNA, (dsRNA) endonucleases. The simplest bacterial enzyme contains an, endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III, can affect RNA structure and gene expression in either of two ways: as a, dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein, that binds but does not cleave dsRNA. We previously determined the endoND, structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a, catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present, the crystal structure of Aa-RNase III in complex with dsRNA, revealing a, noncatalytic assembly. The major differences between the two functional, forms of RNase III.dsRNA are the conformation of the protein and the, orientation and location of dsRNA. The flexibility of a 7 residue linker, between the endoND and dsRBD enables the transition between these two, forms.

Ribonuclease III (RNase III) represents a family of double-stranded RNA (dsRNA) endonucleases. The simplest bacterial enzyme contains an endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III can affect RNA structure and gene expression in either of two ways: as a dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein that binds but does not cleave dsRNA. We previously determined the endoND structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present the crystal structure of Aa-RNase III in complex with dsRNA, revealing a noncatalytic assembly. The major differences between the two functional forms of RNase III.dsRNA are the conformation of the protein and the orientation and location of dsRNA. The flexibility of a 7 residue linker between the endoND and dsRBD enables the transition between these two forms.

==About this Structure==

1RC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with TRS as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1RC7 OCA].

1RC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RC7 OCA].

==Reference==

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[[Category: rna interference]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 01:27:~~20 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:49:19 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1rc7.gif|left|200px]]<br /><applet load="1rc7" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1rc7.gif|left|200px]]<br /><applet load="1rc7" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1rc7, resolution 2.15&Aring;" />
 '''Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution'''<br />
 ==Overview==
-Ribonuclease III (RNase III) represents a family of double-stranded RNA, (dsRNA) endonucleases. The simplest bacterial enzyme contains an, endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III, can affect RNA structure and gene expression in either of two ways: as a, dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein, that binds but does not cleave dsRNA. We previously determined the endoND, structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a, catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present, the crystal structure of Aa-RNase III in complex with dsRNA, revealing a, noncatalytic assembly. The major differences between the two functional, forms of RNase III.dsRNA are the conformation of the protein and the, orientation and location of dsRNA. The flexibility of a 7 residue linker, between the endoND and dsRBD enables the transition between these two, forms.
+Ribonuclease III (RNase III) represents a family of double-stranded RNA (dsRNA) endonucleases. The simplest bacterial enzyme contains an endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III can affect RNA structure and gene expression in either of two ways: as a dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein that binds but does not cleave dsRNA. We previously determined the endoND structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present the crystal structure of Aa-RNase III in complex with dsRNA, revealing a noncatalytic assembly. The major differences between the two functional forms of RNase III.dsRNA are the conformation of the protein and the orientation and location of dsRNA. The flexibility of a 7 residue linker between the endoND and dsRBD enables the transition between these two forms.
 ==About this Structure==
-RC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with TRS as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RC7 OCA].
+RC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RC7 OCA].
 ==Reference==
@@ Line 23: / Line 23: @@
 [[Category: rna interference]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:27:20 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:49:19 2008''