1rm6: Difference between revisions
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New page: left|200px<br /><applet load="1rm6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rm6, resolution 1.60Å" /> '''Structure of 4-hydro... |
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[[Image:1rm6.gif|left|200px]]<br /><applet load="1rm6" size=" | [[Image:1rm6.gif|left|200px]]<br /><applet load="1rm6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1rm6, resolution 1.60Å" /> | caption="1rm6, resolution 1.60Å" /> | ||
'''Structure of 4-hydroxybenzoyl-CoA reductase from Thauera aromatica'''<br /> | '''Structure of 4-hydroxybenzoyl-CoA reductase from Thauera aromatica'''<br /> | ||
==Overview== | ==Overview== | ||
The Mo-flavo-Fe/S-dependent heterohexameric protein complex | The Mo-flavo-Fe/S-dependent heterohexameric protein complex 4-hydroxybenzoyl-CoA reductase (4-HBCR, dehydroxylating) is a central enzyme of the anaerobic degradation of phenolic compounds and belongs to the xanthine oxidase (XO) family of molybdenum enzymes. Its X-ray structure was established at 1.6 A resolution. The most pronounced difference between 4-HBCR and other structurally characterized members of the XO family is the insertion of 40 amino acids within the beta subunit, which carries an additional [4Fe-4S] cluster at a distance of 16.5 A to the isoalloxazine ring of FAD. The architecture of 4-HBCR and concomitantly performed electron transfer rate calculations suggest an inverted electron transfer chain from the donor ferredoxin via the [4Fe-4S] cluster to the Mo over a distance of 55 A. The binding site of 4-hydroxybenzoyl-CoA is located in an 18 A long channel lined up by several aromatic side chains around the aromatic moiety, which are proposed to shield and stabilize the postulated radical intermediates during catalysis. | ||
==About this Structure== | ==About this Structure== | ||
1RM6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thauera_aromatica Thauera aromatica] with CL, K, NA, SO4, PCD, FAD, SF4, FES and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/4-hydroxybenzoyl-CoA_reductase 4-hydroxybenzoyl-CoA reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.20 1.3.99.20] Full crystallographic information is available from [http:// | 1RM6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thauera_aromatica Thauera aromatica] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=PCD:'>PCD</scene>, <scene name='pdbligand=FAD:'>FAD</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=FES:'>FES</scene> and <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/4-hydroxybenzoyl-CoA_reductase 4-hydroxybenzoyl-CoA reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.20 1.3.99.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RM6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Thauera aromatica]] | [[Category: Thauera aromatica]] | ||
[[Category: Boll, M.]] | [[Category: Boll, M.]] | ||
[[Category: Dutton, P | [[Category: Dutton, P L.]] | ||
[[Category: Ermler, U.]] | [[Category: Ermler, U.]] | ||
[[Category: Page, C | [[Category: Page, C C.]] | ||
[[Category: Unciuleac, M.]] | [[Category: Unciuleac, M.]] | ||
[[Category: Warkentin, E.]] | [[Category: Warkentin, E.]] | ||
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[[Category: xanthine oxidase family]] | [[Category: xanthine oxidase family]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:52:21 2008'' | ||
