1sds: Difference between revisions

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[[Image:1sds.gif|left|200px]]<br /><applet load="1sds" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1sds.gif|left|200px]]<br /><applet load="1sds" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1sds, resolution 1.80Å" />

'''Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA'''<br />

==Overview==

The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5, kDa/Snu13 recognize K-turns. This structural motif is canonically, comprised of two stems (one with tandem A.G base pairs, the other with, Watson-Crick pairs) linked by an asymmetric internal loop. L7Ae recognizes, conventional K-turns in ribosomal and box C/D RNAs but also binds, specifically to some box H/ACA RNAs at terminal stem loops. These have the, A.G paired stem, but lack the Watson-Crick stem. The structure of, Methanococcus jannaschii L7Ae bound to a symmetric duplex RNA without, Watson-Crick stems demonstrates how a binding site for this component of, diverse ribonucleoprotein complexes can be constructed with only the A.G, stem and the loop. The RNA adopts a functional conformation with the aid, of a base triple and tight binding of divalent cations. Comparison with, the 15.5 kDa/Snu13-RNA complex structure suggests why the eukaryotic, homolog does not recognize terminal stem loop L7Ae binding sites.

The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5 kDa/Snu13 recognize K-turns. This structural motif is canonically comprised of two stems (one with tandem A.G base pairs, the other with Watson-Crick pairs) linked by an asymmetric internal loop. L7Ae recognizes conventional K-turns in ribosomal and box C/D RNAs but also binds specifically to some box H/ACA RNAs at terminal stem loops. These have the A.G paired stem, but lack the Watson-Crick stem. The structure of Methanococcus jannaschii L7Ae bound to a symmetric duplex RNA without Watson-Crick stems demonstrates how a binding site for this component of diverse ribonucleoprotein complexes can be constructed with only the A.G stem and the loop. The RNA adopts a functional conformation with the aid of a base triple and tight binding of divalent cations. Comparison with the 15.5 kDa/Snu13-RNA complex structure suggests why the eukaryotic homolog does not recognize terminal stem loop L7Ae binding sites.

==About this Structure==

1SDS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with CA and K as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1SDS OCA].

1SDS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDS OCA].

==Reference==

Line 13:

[[Category: Methanocaldococcus jannaschii]]

[[Category: Single protein]]

[[Category: Amare, A.Ferre-D.]]

[[Category: Amare, A Ferre-D.]]

[[Category: Hamma, T.]]

[[Category: CA]]

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[[Category: protein-rna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 02:18:~~18 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:00:28 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1sds.gif|left|200px]]<br /><applet load="1sds" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1sds.gif|left|200px]]<br /><applet load="1sds" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1sds, resolution 1.80&Aring;" />
 '''Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA'''<br />
 ==Overview==
-The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5, kDa/Snu13 recognize K-turns. This structural motif is canonically, comprised of two stems (one with tandem A.G base pairs, the other with, Watson-Crick pairs) linked by an asymmetric internal loop. L7Ae recognizes, conventional K-turns in ribosomal and box C/D RNAs but also binds, specifically to some box H/ACA RNAs at terminal stem loops. These have the, A.G paired stem, but lack the Watson-Crick stem. The structure of, Methanococcus jannaschii L7Ae bound to a symmetric duplex RNA without, Watson-Crick stems demonstrates how a binding site for this component of, diverse ribonucleoprotein complexes can be constructed with only the A.G, stem and the loop. The RNA adopts a functional conformation with the aid, of a base triple and tight binding of divalent cations. Comparison with, the 15.5 kDa/Snu13-RNA complex structure suggests why the eukaryotic, homolog does not recognize terminal stem loop L7Ae binding sites.
+The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5 kDa/Snu13 recognize K-turns. This structural motif is canonically comprised of two stems (one with tandem A.G base pairs, the other with Watson-Crick pairs) linked by an asymmetric internal loop. L7Ae recognizes conventional K-turns in ribosomal and box C/D RNAs but also binds specifically to some box H/ACA RNAs at terminal stem loops. These have the A.G paired stem, but lack the Watson-Crick stem. The structure of Methanococcus jannaschii L7Ae bound to a symmetric duplex RNA without Watson-Crick stems demonstrates how a binding site for this component of diverse ribonucleoprotein complexes can be constructed with only the A.G stem and the loop. The RNA adopts a functional conformation with the aid of a base triple and tight binding of divalent cations. Comparison with the 15.5 kDa/Snu13-RNA complex structure suggests why the eukaryotic homolog does not recognize terminal stem loop L7Ae binding sites.
 ==About this Structure==
-SDS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with CA and K as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SDS OCA].
+SDS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDS OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Methanocaldococcus jannaschii]]
 [[Category: Single protein]]
-[[Category: Amare, A.Ferre-D.]]
+[[Category: Amare, A Ferre-D.]]
 [[Category: Hamma, T.]]
 [[Category: CA]]
@@ Line 19: / Line 19: @@
 [[Category: protein-rna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:18:18 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:00:28 2008''