1sgv: Difference between revisions
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New page: left|200px<br /><applet load="1sgv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sgv, resolution 1.90Å" /> '''STRUCTURE OF TRNA PS... |
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[[Image:1sgv.gif|left|200px]]<br /><applet load="1sgv" size=" | [[Image:1sgv.gif|left|200px]]<br /><applet load="1sgv" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1sgv, resolution 1.90Å" /> | caption="1sgv, resolution 1.90Å" /> | ||
'''STRUCTURE OF TRNA PSI55 PSEUDOURIDINE SYNTHASE (TRUB)'''<br /> | '''STRUCTURE OF TRNA PSI55 PSEUDOURIDINE SYNTHASE (TRUB)'''<br /> | ||
==Overview== | ==Overview== | ||
The three-dimensional structure of the RNA-modifying enzyme, psi55 tRNA | The three-dimensional structure of the RNA-modifying enzyme, psi55 tRNA pseudouridine synthase from Mycobacterium tuberculosis, is reported. The 1.9-A resolution crystal structure reveals the enzyme, free of substrate, in two distinct conformations. The structure depicts an interesting mode of protein flexibility involving a hinged bending in the central beta-sheet of the catalytic module. Key parts of the active site cleft are also found to be disordered in the substrate-free form of the enzyme. The hinge bending appears to act as a clamp to position the substrate. Our structural data furthers the previously proposed mechanism of tRNA recognition. The present crystal structure emphasizes the significant role that protein dynamics must play in tRNA recognition, base flipping, and modification. | ||
==About this Structure== | ==About this Structure== | ||
1SGV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure | 1SGV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry 1S71. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SGV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chan, S.]] | [[Category: Chan, S.]] | ||
[[Category: Chaudhuri, B | [[Category: Chaudhuri, B N.]] | ||
[[Category: Perry, L | [[Category: Perry, L J.]] | ||
[[Category: TBSGC, TB | [[Category: TBSGC, TB Structural Genomics Consortium.]] | ||
[[Category: Yeates, T | [[Category: Yeates, T O.]] | ||
[[Category: hinged motion]] | [[Category: hinged motion]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
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[[Category: trna modification]] | [[Category: trna modification]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:21 2008'' | ||
