1sjj: Difference between revisions
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'''Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin'''<br /> | '''Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin'''<br /> | ||
==Overview== | ==Overview== | ||
Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm | Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm resolution of 2-D arrays of smooth muscle alpha-actinin. The reconstruction reveals a well-resolved long central domain with 90 degrees of left-handed twist and near 2-fold symmetry. However, the molecular ends which contain the actin binding and calmodulin-like domains, have different structures oriented approximately 90 degrees to each other. Atomic structures for the alpha-actinin domains were built by homology modeling and assembled into an atomic model. Model building suggests that in the 2-D arrays, the two calponin homology domains that comprise the actin-binding domain have a closed conformation at one end and an open conformation at the other end due to domain swapping. The open and closed conformations of the actin-binding domain suggests flexibility that may underlie Ca2+ regulation. The approximately 90 degrees orientation difference at the molecular ends may underlie alpha-actinin's ability to crosslink actin filaments in nearly any orientation. | ||
==About this Structure== | ==About this Structure== | ||
1SJJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http:// | 1SJJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJJ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
[[Category: Taylor, D | [[Category: Taylor, D W.]] | ||
[[Category: Taylor, K | [[Category: Taylor, K A.]] | ||
[[Category: 3-helix bundle]] | [[Category: 3-helix bundle]] | ||
[[Category: actin binding protein]] | [[Category: actin binding protein]] | ||
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[[Category: calponin homology domain]] | [[Category: calponin homology domain]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:10 2008'' | ||
Revision as of 13:02, 21 February 2008
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Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin
Overview
Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm resolution of 2-D arrays of smooth muscle alpha-actinin. The reconstruction reveals a well-resolved long central domain with 90 degrees of left-handed twist and near 2-fold symmetry. However, the molecular ends which contain the actin binding and calmodulin-like domains, have different structures oriented approximately 90 degrees to each other. Atomic structures for the alpha-actinin domains were built by homology modeling and assembled into an atomic model. Model building suggests that in the 2-D arrays, the two calponin homology domains that comprise the actin-binding domain have a closed conformation at one end and an open conformation at the other end due to domain swapping. The open and closed conformations of the actin-binding domain suggests flexibility that may underlie Ca2+ regulation. The approximately 90 degrees orientation difference at the molecular ends may underlie alpha-actinin's ability to crosslink actin filaments in nearly any orientation.
About this Structure
1SJJ is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region., Liu J, Taylor DW, Taylor KA, J Mol Biol. 2004 Apr 16;338(1):115-25. PMID:15050827
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