1smg: Difference between revisions

Line 1:

[[Image:1smg.gif|left|200px]]<br /><applet load="1smg" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1smg.gif|left|200px]]<br /><applet load="1smg" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1smg" />

'''CALCIUM-BOUND E41A MUTANT OF THE N-DOMAIN OF CHICKEN TROPONIN C, NMR, 40 STRUCTURES'''<br />

==Overview==

The structural transition in troponin C induced by the binding of two, calcium ions involves an "opening" of the structure, an event that, triggers skeletal muscle contraction. We have solved the solution, structure of a mutant (E41A) of the regulatory domain of skeletal troponin, C wherein one bidentate ligand to the calcium in site I is missing. This, structure remains "closed" upon calcium binding, indicating that the, linkage between calcium binding and the induced conformational change has, been broken. This provides a snapshot of skeletal troponin C between the, off and on state and thereby valuable insight into the mechanism of, regulation within skeletal TnC. Although several factors contribute to the, triggering mechanism, the opening of the troponin C structure is, ultimately dependent on one amino acid, Glu41. Insights into the structure, of cardiac troponin C can also be derived from this skeletal mutant.

The structural transition in troponin C induced by the binding of two calcium ions involves an "opening" of the structure, an event that triggers skeletal muscle contraction. We have solved the solution structure of a mutant (E41A) of the regulatory domain of skeletal troponin C wherein one bidentate ligand to the calcium in site I is missing. This structure remains "closed" upon calcium binding, indicating that the linkage between calcium binding and the induced conformational change has been broken. This provides a snapshot of skeletal troponin C between the off and on state and thereby valuable insight into the mechanism of regulation within skeletal TnC. Although several factors contribute to the triggering mechanism, the opening of the troponin C structure is ultimately dependent on one amino acid, Glu41. Insights into the structure of cardiac troponin C can also be derived from this skeletal mutant.

==About this Structure==

1SMG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1SMG OCA].

1SMG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMG OCA].

==Reference==

Line 13:

[[Category: Gallus gallus]]

[[Category: Single protein]]

[[Category: Gagne, S.M.]]

[[Category: Gagne, S M.]]

[[Category: Li, M.X.]]

[[Category: Li, M X.]]

[[Category: Sykes, B.D.]]

[[Category: Sykes, B D.]]

[[Category: CA]]

[[Category: calcium-binding protein]]

Line 21:

[[Category: troponin c]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 02:29:~~07 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:03:02 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1smg.gif|left|200px]]<br /><applet load="1smg" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1smg.gif|left|200px]]<br /><applet load="1smg" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1smg" />
 '''CALCIUM-BOUND E41A MUTANT OF THE N-DOMAIN OF CHICKEN TROPONIN C, NMR, 40 STRUCTURES'''<br />
 ==Overview==
-The structural transition in troponin C induced by the binding of two, calcium ions involves an "opening" of the structure, an event that, triggers skeletal muscle contraction. We have solved the solution, structure of a mutant (E41A) of the regulatory domain of skeletal troponin, C wherein one bidentate ligand to the calcium in site I is missing. This, structure remains "closed" upon calcium binding, indicating that the, linkage between calcium binding and the induced conformational change has, been broken. This provides a snapshot of skeletal troponin C between the, off and on state and thereby valuable insight into the mechanism of, regulation within skeletal TnC. Although several factors contribute to the, triggering mechanism, the opening of the troponin C structure is, ultimately dependent on one amino acid, Glu41. Insights into the structure, of cardiac troponin C can also be derived from this skeletal mutant.
+The structural transition in troponin C induced by the binding of two calcium ions involves an "opening" of the structure, an event that triggers skeletal muscle contraction. We have solved the solution structure of a mutant (E41A) of the regulatory domain of skeletal troponin C wherein one bidentate ligand to the calcium in site I is missing. This structure remains "closed" upon calcium binding, indicating that the linkage between calcium binding and the induced conformational change has been broken. This provides a snapshot of skeletal troponin C between the off and on state and thereby valuable insight into the mechanism of regulation within skeletal TnC. Although several factors contribute to the triggering mechanism, the opening of the troponin C structure is ultimately dependent on one amino acid, Glu41. Insights into the structure of cardiac troponin C can also be derived from this skeletal mutant.
 ==About this Structure==
-SMG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SMG OCA].
+SMG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMG OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Gallus gallus]]
 [[Category: Single protein]]
-[[Category: Gagne, S.M.]]
+[[Category: Gagne, S M.]]
-[[Category: Li, M.X.]]
+[[Category: Li, M X.]]
-[[Category: Sykes, B.D.]]
+[[Category: Sykes, B D.]]
 [[Category: CA]]
 [[Category: calcium-binding protein]]
@@ Line 21: / Line 21: @@
 [[Category: troponin c]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:29:07 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:03:02 2008''